植物△1-吡咯啉-5-羧酸合成酶（P5CS）基因的研究进展  

Advance in △1-pyrroline-5-carboxylate synthase （P5CS） gene in plants

作　　者：李健[1,2] 彭东海[1,2] 张风娟[1,2] 杨丽涛[1,2,3] 李杨瑞[1,2,3,4] 

机构地区：[1]广西大学农学院,南宁530005 [2]亚热带农业生物资源保护与利用国家重点实验室,南宁530005 [3]中国农业科学院甘蔗研究中心/农业部广西甘蔗生物技术与遗传改良重点实验室/广西甘蔗遗传改良重点实验室,南宁530007 [4]广西农业科学院,南宁530007

出　　处：《南方农业学报》2014年第9期1545-1550,共6页Journal of Southern Agriculture

基　　金：国家"863"计划项目(2013AA102604);国家国际科技合作专项项目(2013DFA31600);广西自然科学基金项目(2013NXNSFAA019073);广西八桂学者和特聘专家专项基金项目([2013]3号)

摘　　要：△1-吡咯啉-5-羧酸合成酶(P5CS)是植物脯氨酸生物合成的关键酶。近年来,许多学者对P5CS酶基因克隆、其在植物中的表达特性与在抗逆基因工程上的应用进行了大量研究,证实P5CS基因已成功在许多植物中进行过量表达并能有效提高植物的抗旱、耐盐性。随着分子生物学和现代生物技术的快速发展,今后可进一步挖掘利用植物P5CS基因,有效验证其功能和了解其作用机制,探讨P5CS基因与其他基因的互作及调控植物抗逆的生理生化机理,研究其在植物生长发育及器官发生等过程中的作用;培育出高效、实用、抗逆性强的转基因植物品种,均有待今后进行深入研究。△1-pyrroline-5-carboxylate synthase （P5CS） is a key enzyme in plant proline biosynthesis. In recent years, P5CS gene cloning and its expression characteristic and application on stress resistance genetic engineering were studied widely. PSCS gene has been proved to be successfully overexpressed in many plants and could improve plant drought resistance and salt-tolerance. With the rapid development of molecular biology and modern biotechnology, P5CS gene in more plants should be studied to verify its function and role mechanism. The interaction of P5CS gene with other genes and their physiological-chemical mechanism on regulating plant resistant stress should be explored. The role of P5CS gene on growth and develcpment, and organogenesis of plant should be further studied. Further- more, transgenic plant varieties with high-efficiency, practical and strong stress resistance should be bred to improve plants resistant to adversity stress in the future.

关 键 词：P5CS基因 脯氨酸 渗透胁迫 基因工程 

分 类 号：Q943.2[生物学—植物学]