3-氨基苯甲酰胺减轻骨骼肌缺血再灌注损伤的实验研究  

3-aminobenzamide Attenuates Ischemia Reperfusion Injury of Skeletal Muscle

作　　者：田保玲[1] 李奇[2] 纪莲[1] 刘晓梅 杨向红[1] 

机构地区：[1]中国医科大学附属盛京医院病理科,沈阳110004 [2]沈阳医学院附属中心医院手外三科,沈阳110024

出　　处：《中国医科大学学报》2014年第10期906-909,共4页Journal of China Medical University

基　　金：辽宁省教育厅高校科研计划(L2010574)

摘　　要：目的研究3-氨基苯甲酰胺(3AB)对鼠骨骼肌缺血再灌注损伤的保护作用,以及组织中凋亡和胀亡的存在情况。方法54只SD大鼠随机分为空白对照组、缺血再灌注组、3AB组,持续缺血4 h,再灌注6、24和48 h。检测血浆乳酸脱氢酶(LDH)、肌酸磷酸激酶(CPK)活性,肌肉内丙二醛(MDA)含量、总超氧化物歧化酶(SOD)活性,进行组织学、免疫组化、超微结构分析。结果3AB组在再灌注24 h和48 h时,在MDA含量下降、SOD活性升高、骨骼肌湿重与干重比值下降、组织学改变范围及免疫组化阳性范围方面,均与缺血再灌注组有明显差异。结论再灌注开始时应用3AB对于缺血再灌注损伤有明显的保护作用,主要体现在再灌注的稍后期阶段(再灌注24 h和48h)。缺血再灌注过程中,凋亡和胀亡是并存的。Objective To investigate the protection effect of 3-aminobenzamide （3AB）, and the existence of apoptosis and oncosis in ischemia re- peffusion injury of skeletal muscle. Methods Totally 54 adult SD rats were randomty divided into control groups, ischemia reperfusion groups, and 3AB groups, which underwent 4 h of ischemia and different phases of repeffusion. The lactic dehydrogenase （LDH）, creatine phosphokinase （CPK）, superoxide dismutase （SOD） activity, malondiadehyde （MDA） content were determined. The changes of histology, immunohistology, and uhramicmstrucmre were observed. Results Compared with ischemia reperfusion groups, 3AB groups showed obvious differences in MDA content, SOD activity, wet-to-dry weight ratio, histological and immtmohistological changes. Condusion 3-aminobenzamide has protected effects on isch- emia repeffusion of skeletal muscle. Apoptosis and oncosis coexist during the ischemia reperfusion injury.

关 键 词：缺血再灌注损伤 3-氨基苯甲酰胺 骨骼肌 

分 类 号：R364.1[医药卫生—病理学]