盐酸二氧丙嗪及其与β-环糊精包络作用的荧光分析法研究  

Study on Fluorescence Analysis of Dioxopromethazine and Its Inclusion Reaction with β-Cyclodextrin

作　　者：施红[1] 刘娥[1] 张华新[1] 

机构地区：[1]荆楚理工学院化工与药学院,湖北荆门448000

出　　处：《化学与生物工程》2014年第10期47-50,共4页Chemistry & Bioengineering

基　　金：药物合成与优化湖北省重点实验室开放基金资助项目(OPP2014YB01);荆楚理工学院科研项目(ZR201302;ZR201317)

摘　　要：采用荧光光谱法研究了盐酸二氧丙嗪(DOPM)的荧光现象及其与β-环糊精(β-CD)的包络作用,发现当β-CD与DOPM浓度比大于10 000∶1时,β-CD对DOPM有一定的荧光增敏作用,当β-CD与DOPM浓度比小于5 000∶1时,β-CD对DOPM有较强的荧光猝灭作用;提出了测定DOPM含量的荧光光谱分析新方法,该法的检出限为1.001×10-7 mol·L-1,平均回收率为99.1%;利用Lineweaver-Burk双倒数方程和Benesi-Hildebrand方程分别处理实验数据,得到了DOPM与β-CD包络作用的结合常数和热力学参数,并对包络作用机理进行了初步探讨。Fluorescence of dioxopromethazine（DOPM）and its inclusion reaction withβ-cyclodextrin（β-CD） were studied by fluorescence analysis.Results showed that,when concentration ratio ofβ-CD to DOPM was more than 10 000∶1,β-CD could enhance the fluorescence intensity of DOPM and when concentration ratio ofβ-CD to DOPM was lower than 5 000∶1,β-CD had quenching effect on the fluorescence intensity of DOPM.The novel detection method for DOPM content with detection limit of 1.001×10-7 mol·L-1 and average recovery rate of 9 9.1% was established.The experimental data were transacted by Lineweaver-Burk double-reciprocal e-quation and Benesi-Hildebrand equation.The binding constant and thermodynamic parameters were calculated according to both equation.The mechanism of inclusion reaction was preliminarily discussed as well.

关 键 词：盐酸二氧丙嗪 Β-环糊精 包络作用 荧光分析 

分 类 号：O657.31[理学—分析化学]