大连海域沉积物中可培养海洋链霉菌活性及其多样性  

Activity and diversity of culturable Streptomyces isolated from marine sediments of sea areas of Dalian

作　　者：于基成[1] 刘秋[1] 邵阳[1] 刘长建[1] 闫建芳[1] 齐小辉[1] 

机构地区：[1]大连民族学院生命科学学院,大连116600

出　　处：《生态学报》2014年第20期5896-5906,共11页Acta Ecologica Sinica

基　　金：国家自然科学基金(31070005;31270057);中央高校基本科研业务费专项资金资助(DC12010103)

摘　　要：以大肠杆菌、金黄色葡萄球菌和尖孢镰刀枯萎病菌作为测试靶目标,采用9种分离培养基从大连海域13个不同采样点的海洋沉积物样品中分离到165株海洋链霉菌。从165株海洋放线菌中筛选到对金黄色葡萄球菌具有抑制活性的菌株85株,占总菌株数的51.5%;对大肠杆菌具有抑制活性的菌株27株,占总菌株数的16.4%;对尖孢镰刀枯萎病菌具有抑制活性的菌株仅有6株,占总菌株数的3.6%。因此,海洋链霉菌的活性更多地表现为对细菌的抗性,尤其对革兰氏阳性细菌具有更高的抑制活性。对其中具有抑制活性或形态独特的菌株进行了16S r DNA序列分析,并构建系统发育树,显示活性海洋链霉菌具有丰富的种类多样性和广谱抗菌活性。同种海洋链霉菌与土壤链霉菌活性比较结果也表明,海洋链霉菌多表现抗革兰氏阳性细菌活性。Actinomycetesare an important source of microorganisms used in medicine. Tens of thousands of biologically active substances of microbial origin have been found,about 45% of which are produced by actinomycetes. The marine environment has high salinity,high pressure,low temperature,low nutritive content and other characteristics,so that the population characteristics and metabolic pathways of marine actinomycetes are different from those of terrestrial actinomycetes,and can be a source of novel species and metabolites. In total,165 antimicrobial isolates were screened from13 marine sediment samples collected in the seas around Dalian using nine different media. The results showed that improved humic acid vitamin agar medium（ HV-2） and glycerol arginine agar medium（ M2） were the best types of medium for isolating marine actinomycetes,followed by improved LSE-SE-2 agar medium（ LSE-SE-2） and oat agar medium（ OM）.Using agar block methods,the antagonistic activity of the 165 strains against three pathogens（ Fusariumoxysporium f sp.cucumarinum,Staphylococcus aureus and Escherichia coli） was analyzed in vitro. Among the 165 isolates,95 strains showed antagonistic activity against these three test pathogens. Of the 165 isolates,85（ 51.5%） strains showed activity against S.aureus. The diameter of the inhibition zone of four of the 85 strains was more than 30 mm; 27 strains（ 16.4% of the total isolates） showed activity against E. coli,and the diameter of the inhibition zone of strains YH6 and YH21 against E. coli was more than 27 mm. Six strains（ M95,M108,HV14,HL9,HE16,HE66）,3. 6% of the total isolates,showed activity against F. oxysporumf sp. cucumarinum. The results indicated that marine actinomycetes often show antagonistic activity against bacteria,especially Gram-positive bacteria.Meanwhile,16 S r DNA sequences of interesting strains with either specialphenotypes or strong antimicrobial activity were analyzed. Phylogenetic analysis using 16 S r DNA sequencing revealed that marine Strepto

关 键 词：海洋链霉菌 种群多样性 活性 

分 类 号：Q178.53[生物学—水生生物学]