宫颈癌患者HPV感染和调节性T细胞的相关性研究  

Study on the relationship of human papilloma virus and TReg in cervical cancer patients

作　　者：刘志辉[1] 全超[1] 宋明旭[1] 

机构地区：[1]江南大学附属医院肿瘤研究所,无锡214062

出　　处：《国际遗传学杂志》2014年第5期216-219,244,共5页International Journal of Genetics

基　　金：基金项目：无锡医院管理中心科研项目（YGZXQ1303）

摘　　要：目的 探讨宫颈部位人乳头瘤病毒（HPV）感染与CD4＋ CD25＋ CD127-调节性T细胞（TReg）及机体免疫水平的关系.方法 采用第二代核酸杂交扩增技术（HC2）检测41例正常对照组、50例宫颈上皮内瘤变（CIN）Ⅰ、42例CIN Ⅱ～Ⅲ和70例感染HPV宫颈癌患者;采用流式细胞术检测外周血CD4＋CD25＋ CD127-TReg、细胞毒性T细胞（CTL）和NK百分率;采用酶联免疫吸附实验（ELISA）检测血清中TGF-β1和INF-γ的含量.结果 ①HPV感染率随着宫颈病变的加重而升高（P =5.75×10^-19）;②宫颈CIN Ⅱ～Ⅲ组与宫颈癌组外周血CD4＋CD25＋CD127-TReg、CTL、NK、TGF-β1、INF-γ与CIN Ⅰ组及正常对照组比较,差异均 有统计学意义（P=1.03×10^-9）;且在HPV阴阳性组间比较亦有统计学意义（P=2.33×10^-4）;③CTL与NK和Treg呈负相关（P值分别为1.62×10^-6和3.41×10^-5）;INF-γ含量与TReg呈负相关（P=2.11×10^-5）;④HPV与CD4＋ CD25＋ CD127-TReg百分率呈正相关（P =3.02×10^-6）.结论 宫颈HPV感染与TReg密切相关,TReg失调导致的免疫水平下降可能是宫颈癌免疫逃避机制之一.Objective To investigate the relationship between human papilloma virus （HPV） cervical infection and the level of CD4＋ CD25＋ CD127- regulatory T cells （TReg）. Methods HPV was evaluated b.y nucleic acid amplification typing kit in 41 normal control group, 50 cervical intraepithelial neoplasia （ CIN ） Ⅰ , 42 CIN Ⅱ-Ⅲ and 70 cervical cancer patients with HPV infection. The percentage of TReg population of CD4 ＋ T cells, CTL and NK were evaluated by flow cytometric analysis. The levels of TGF- β and INF - γ in serum were detected by enzyme-linked immunosorbent assay （ ELISA ） . Results （1）Infeetion rate of HPV tended to increase with the aggravation of cervical lesion （ P = 5.75 × 10^-19） ; （2）The percentage of CD4＋ CD25＋ CD127- TReg, CTL, NK, TGF-β1 , INF-γ inperipheral blood from patients with cervical cancer and CIN Ⅱ-Ⅲ were significantly changed in comparison with that in patients with CIN I and in normal controls （ P = 1.03 × 10^-9 ） , and there was a statistical difference between HPV positive and negatively groups （P = 2.33 × 10^-4 ） ; （2）CTL and .NK were negatively correlated with TReg （ P = 1.62 × 10^-6 , P = 3.41×10^-5 ） , the level of INF-3, was negatively correlated with TReg （P =2. 11 × 10^-5 ） ; （4）CD4＋ CD25＋ CD127- TReg was positive correlated with HPV infec-tion （ P = 3.02 ×10^-6 ）. Conclusion The HPV infection in cervix is intimately correlated with TReg, TReg imbalance may be one of the mechanisms underlying immune escape in cervical cancer patients.

关 键 词：宫颈癌 人乳头瘤病毒 调节性T细胞 

分 类 号：R737.33[医药卫生—肿瘤]