野韭菜花器官脱分化影响因子研究  

Research on Factors of the Dedifferentiation of Floral Organ in Allium hookeri Thwaites

作　　者：王茜[1] 龙骊丰 韦鹏霄[1] 岑秀芬[1] 万正林[1,3] 龙明华[1] 

机构地区：[1]广西大学农学院,广西南宁530004 [2]广西大学行健文理学院理工学部,广西南宁530005 [3]广西现代农业科技示范园,广西南宁530007

出　　处：《安徽农业科学》2014年第31期10861-10863,共3页Journal of Anhui Agricultural Sciences

基　　金：国家现代农业产业技术体系广西大宗蔬菜创新团队专项资基项目(nycytxgxcxtd-03-10-1);广西自然科学基金项目(2011GXNSFB018033);广西科技攻关项目(桂科攻0323001-1);广西农业科学院科技发展基金项目(201001);广西南宁科技局项目(201102057B)

摘　　要：[目的]研究影响野韭菜花器官脱分化的诱导因子。[方法]将野韭菜花器官进行离体培养,探讨培养基种类、外源激素种类和浓度及配比、花器官不同发育时期及不同部位对愈伤组织诱导的影响。[结果]MS基本培养基最适合花蕾愈伤组织的诱导;NAA比其他生长素更有利于提高花蕾的岀愈率;花苞未开状态的花蕾愈伤组织诱导率最高;愈伤组织发生具有明显的部位效应,最好是花盘,其次是花蕾,较差是花柄,而花茎无愈伤组织发生。[结论]为建立野韭菜组织培养快繁体系奠定基础。[ Objective ] The aim was to study factors of the differentiation of floral organ in Allium hookeri Thwaites. [ Method ] Using flower organ of Allium hookeri Thwaites as explants, the influence of different factors, including medium variety, the concentration and ingredient of exogenous hormones and the different growth stages of flower organ were investigated. [ Result ] The results revealed that MS basic medium was optimum for callas induction. Among those auxins, NAA was the best to improve the induction rate of flower buds, and the callus induction showed the highest rate in the unopened flower buds. The study also demonstrated that the callus induction was obvious distinguishing in different flower positions. The callus of flower disc was better than bud, while pedicel showed the lower rate in callus induction and the floral axis had no dedifferentiation. [ Conclusion] The study laid technology foundation for establishing tissue culture rapid propagation system of AUium hookeri Thwaites.

关 键 词：野韭菜 愈伤组织诱导 花器官 

分 类 号：S633.3[农业科学—蔬菜学]