超声法优化金叶女贞叶中总黄酮的提取  

Optimum Extraction of Total Flavonoids from Ligustrum Lucidum cv.by Ultrasonic Wave

作　　者：王建安[1] 付英杰[1] 李艳芝[1] 雷绘敏 陈庆红[1] 

机构地区：[1]济宁医学院药学院,山东日照276826

出　　处：《南京师大学报（自然科学版）》2014年第3期106-110,共5页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：日照市科技发展项目(2011sf 006)

摘　　要：以金叶女贞叶为材料,寻找其中总黄酮的超声提取的最佳条件.以总黄酮含量为考察指标,在单因素实验的基础上,采用L9(34)正交实验,超声提取研究了乙醇浓度、超声提取时间、超声功率、超声温度对提取金叶女贞叶中总黄酮的影响.从实验结果分析,影响超声提取效果的因素为乙醇浓度>超声时间>提取功率>提取温度,结果表明粒度为40目,乙醇浓度为65%,超声时间为75 min,超声功率为400 W,提取温度为50℃时总黄酮得率最高.An ultrasonic method for the extraction of total flavonoids from Ligustrum Vicaryi Hort. was investigated and optimized. Using single factor and L9（ 3^4） orthogonal design, the effects of ethanol concentrations, ultrasonic time, ultrasonic power and extraction temperature were considered. The impact will activate by ethanol concentrations, ultrasonic time, ultrasonic power and extraction temperature. The optimum extracting conditions of total flavonoids are ： mesh number 40, extraction time 75 min, extraction temperature 50 ℃, ultrasonic power 400 W and ethanol concentrations 65%. Under these conditions the yield of total flavonoids can reach the highest.

关 键 词：金叶女贞 总黄酮 超声辅助提取 正交设计 

分 类 号：R931.6[医药卫生—生药学]