糖尿病肾病大鼠血浆差异表达蛋白的质谱分析  

Mass spectrometry analysis of differentially expressed proteins in plasma from rats with diabetic nephropathy

作　　者：魏敏[1] 罗仁[2] 李璟怡[1] 柳志伟[1] 郑雪花[1] 

机构地区：[1]福建中医药大学中西医结合学院,福州350122 [2]南方医科大学中医药学院

出　　处：《山西医科大学学报》2014年第10期925-928,共4页Journal of Shanxi Medical University

基　　金：福建省自然科学基金资助项目(2012J01391)

摘　　要：目的探讨应用比较蛋白质组学分析糖尿病肾病大鼠血浆的差异表达蛋白,寻找和糖尿病肾病发生相关的蛋白质。方法建立糖尿病肾病(DN)大鼠模型,将DN模型组和正常组大鼠血浆采用双向凝胶电泳(2-DE)技术分离血浆蛋白,经PD-Quest软件分析结合人工对比确认差异蛋白点,MALDI-TOF/TOF鉴定差异表达的蛋白。结果成功建立2-DE血浆蛋白图谱,经质谱鉴定出8种差异蛋白,这些差异蛋白主要与氧化应激、炎症反应及脂质代谢紊乱等相关。结论双向电泳质谱技术可有效筛选糖尿病肾病相关蛋白,为进一步早期诊断提供依据。Objective To observe the differentially expressed proteins from plasma of rats with diabetic nephropathy by comparative proteomics,and find out the DN-related proteins. Methods Rats were injected with STZ to induce diabetic nephropathy（DN） model. Total proteins in plasma were separated by two-dimensional gel electrophoresis（2-DE） in normal control group and model group. The differential protein spots were confirmed by PD-Quest software combined with artificial comparative analysis, and identified by MALDI- TOF/TOF. Results The 2-DE protein profiles of plasma were successfully established. Eight differentially expressed proteins were identified using MALDI-TOF/TOF, which were mainly involved in oxidative stress, inflammation and lipid metabolism disorders. Conclusion Two-dimensional electrophoresis and mass spectrometry can effectively screen the DN-related proteins, and also provide a basis for further early diagnosis.

关 键 词：糖尿病肾病 双向电泳 质谱 差异蛋白 

分 类 号：R587.2[医药卫生—内分泌]