急性白血病患者外周血IL-2、IFN-γ、IL-10水平及血清LDH活性的研究  

Clinical research on IL-2,IFN-γ,IL-10 and LDH in patients with acute leukemia

作　　者：朱岚[1] 申卫红[1] 龚芳[1] 

机构地区：[1]南通大学第三附属医院检验科,江苏无锡214041

出　　处：《国际检验医学杂志》2014年第19期2593-2594,共2页International Journal of Laboratory Medicine

基　　金：无锡市医院管理中心医学技术重大扶持项目(YGZF1101)

摘　　要：目的探讨急性白血病患者外周血白细胞介素(IL)-2、干扰素-γ(IFN-γ)、IL-10水平及血清乳酸脱氢酶(LDH)活性的变化。方法将65例急性白血病患者分为未缓解组(35例)和缓解组(30例),另选取健康体检者50例为对照组。采用酶联免疫吸附试验(ELISA)检测外周血IL-2、IFN-γ和IL-10的水平,采用全自动生化分析仪检测血清中LDH的活性。结果未缓解组IL-2和IFN-γ水平均明显低于对照组和缓解组(P<0.05),而IL-10水平则明显高于对照组和缓解组(P<0.05);未缓解组血清中LDH活性明显高于对照组和缓解组,差异有统计学意义(P<0.05)。结论急性白血病患者外周血中IL-2、IFN-γ、IL-10水平及血清中LDH活性会发生明显变化,临床上可以通过检测IL-2、IFN-γ、IL-10水平及LDH活性对急性白血病患者进行早期诊断。Objective To study the levels of interleukin（IL）-2 ,interferon-γ（IFN-γ） and IL-10 in peripheral blood and the effec-tive concentration of lactate dehydrogenase（LDH） in serum of patients with acute leukemia .Methods 65 patients with acute leuke-mia were divided into non-remission group（n=35） and remission group（n=30） .50 healthy volunteers were used as control group . The levels of IL-2 ,IFN-γ ,IL-10 in peripheral blood were measured by enzyme linked immunosorbent assay（ELISA） .The effective concentration of LDH was measured by automatic biochemical analyzer .Results The levels of IL-2 ,IFN-γin non-remission group were significantly decreased compared with control group and remission group（P〈0 .05） ,but the level of IL-10 in non-remission group was significantly increased compared with control group and remission group（P〈0 .05） .The effective concentration of LDH in non-remission group was significantly higher than that in control group or remission group（P〈0 .05） .Conclusion The levels of IL-2 ,IFN-γand IL-10 in peripheral blood and the effective concentration of LDH in serum of patients with acute leukemia have changed .Clinical detection of IL-2 ,IFN-γ,IL-10 and LDH may contribute to the early diagnosis of patients with acute leukemia .

关 键 词：急性白血病 白细胞介素-2 白细胞介素-10 干扰素-Γ 

分 类 号：R733.71[医药卫生—肿瘤]