转录因子特化蛋白1在大肠癌组织中的表达及意义  

Expression and clinical significance of transcription factor special protein 1 in colorectal cancer

作　　者：苗晋华[1] 杜叶平[1] 尹莉莉[1] 武春梅[1] 徐丽萍[1] 

机构地区：[1]中国人民解放军第二六四医院检验科,山西太原030001

出　　处：《国际检验医学杂志》2014年第19期2597-2598,共2页International Journal of Laboratory Medicine

基　　金：山西省自然科学基金资助项目(2013011043-4)

摘　　要：目的研究转录因子特化蛋白1(Sp1)在大肠癌组织中的表达情况及意义。方法取60例大肠癌患者的癌组织和癌旁正常组织,分别采用实时定量PCR法检测大肠癌和癌旁正常组织中Sp1mRNA表达情况。按ΔΔCT法对目的基因进行相对定量。比较Sp1mRNA的表达情况与不同临床资料、病理参数之间的关系。结果大肠癌组织中Sp1mRNA表达水平明显高于癌旁正常组织(P<0.01);大肠癌组织中Sp1mRNA阳性表达率与患者的年龄、性别、肿瘤部位无关(P>0.05),而与大肠癌的组织学分级、Duke′s分期、淋巴结转移有关(P<0.05)。结论 Sp1在大肠癌组织中呈现高表达状态,提示Sp1在大肠癌的发生、发展过程中可能起重要作用。Objective To study the expression of transcription factor special protein 1（Sp1） in colorectal cancer tissues and the relationship with the biological behavior .Methods The Sp1 mRNA expressions of 60 colon cancer tissues and their corresponding normal tissues were detected by real-time PCR ,and the level of target gene was calculated by ΔΔCT method .The relationships be-tween the expression of Sp1 mRNA and the different clinical features and pathological characters were determined .Results Com-pared with the matched normal tissues ,Sp1 mRNA was significantly up-regulated in the colon cancer tissues（P〈0 .01） .Sp1 mRNA positive expression rate in colon cancer tissues had no significant different with sex ,age and tumors area（P〉0 .05） ,but had signifi-cant different with histological grade ,Duke′s stages and lymph node metastasis（P〈0 .05） .Conclusion Sp1 plays an important role in the process of occurrence and development in colon cancer .

关 键 词：转录因子特化蛋白1 大肠癌 淋巴结转移 

分 类 号：R735.34[医药卫生—肿瘤]