三氧化二砷对肠癌HCT116细胞Smads表达的影响  

The Effect of Arsenic Trioxide on the Expression of Smads Genes in Intestinal Cancer Cell HCT116

作　　者：冯越[1] 蔡士昌 蔡朋朋[2] 李雪松[2] 孙智睿[3] 费洪新[4] 徐广有[5] 

机构地区：[1]齐齐哈尔医学院 [2]齐齐哈尔医学院附属三院消化科,黑龙江齐齐哈尔161000 [3]齐齐哈尔医学院附属三院心内科,黑龙江齐齐哈尔161000 [4]齐齐哈尔医学院组胚室,黑龙江齐齐哈尔161000 [5]齐齐哈尔医学院微形态实验室,黑龙江齐齐哈尔161000

出　　处：《中外医疗》2014年第23期64-66,共3页China & Foreign Medical Treatment

基　　金：2012年黑龙江省教育厅项目(12521634);2013年黑龙江省大学生创新创业项目(201311230020)

摘　　要：目的 研究三氧化二砷对肠癌细胞HCT116中TGF-β/Smads信号通路的Smads相关基因表达变化,阐述Smads相关基因在肠癌HCT116细胞中的变化规律,探讨肠癌发生的病理机制,和分析As2O3作用新靶点。方法 实验分空白对照组,低剂量As2O3组,中剂量As2O3组,高剂量As2O3组,在体外培养72 h后取出细胞用于实验。采用免疫荧光化学术和Westernblotting检测Smad3、Smad7蛋白的表达变化。结果 三氧化二砷为0.5~2.5μmol/L时,Smad3分别为（19.94±1.57）,（34.17±1.28）,（39.64±1.63）,Smad7分别为（31.53±0.67）,（21.69±0.94）,（20.04±0.31）,与对照组Smad3（16.61±0.64）,Smad7 3（8.61±0.64）比较,各组差异有统计学意义（P〈0.05）;westernblotting显示三氧化二砷能同时下调Smad7蛋白的表达,上调Smad3蛋白的表达而对肿瘤细胞的生长进行调控。结论 TGF-β/Smads信号通路的Smad3,7在肠癌发生发展起重要的调控作用,三氧化二砷可通过改变Smad3,7表达,而对肠癌细胞HCT116生长进行抑制。Objective To study the effect of arsenic trioxide on the expression changes of Smads genes associated with TGF-beta / Smads signaling pathway of intestinal cancer cell HCT116, elaborate the change regulation of Smads genes in intestinal cancer cell HCT116, explore the pathological mechanism of intestinal cancer and analyze the new targets of As203. Methods The cells were divided into blank control group, low dose As203 group, medium dose As203 group, high dose As203 group, and they were used in experiments after 72 hours in culture. Immunofluorescence technique and Westernblotting were used to measure the expression change of Smad3, Smad7 protein. Results Arsenic trioxide was from 0.5 μmol/L to 2.5 μmol/L, Smad3 was respectively 19.94±1.57, 34.17±1.28, 39.64±1.63, Smad7 was respectively 31.53±0.67, 21.69±0.94, 20.04±0.31, compared with the control group＇s （Smad3 16.61±0.64, Smad7 38.61±0.64）, the differences were statistically significant （P〈0.05）. Westernblotting showed that arsenic trioxid .could downregulate the expression of Smad7, at the same time, upregulate the expression of Smad3 protein in tumor cell growth regulation. Conclusion Smad3, 7 of TGF-[3/Smads signaling pathway in intestinal cancer play an important regulating role in the development of intestinal cancer, arsenic trioxide can inhibit HCT116 cells growth by changing the Smad3, 7 expression.

关 键 词：三氧化二砷 HCT116 SMAD3 SMAD7 

分 类 号：R735[医药卫生—肿瘤]