杨树嫩茎质外体微透析液中K^+,Na^+,Ca^(2+)的分析  

Determination of K^+,Na^+ and Ca^(2+) in Miorodialysis of Apoplast of Poplar Stems by Atomic Absorption Spectrometry with Microdialysis Method

作　　者：马金龙[1,2] 姜国斌[3] 姚善泾[1] 姜波[2] 金华[3] 

机构地区：[1]浙江大学化学工程与生物工程学系,杭州310027 [2]大连民族学院生命科学学院生物工程系,大连116600 [3]大连民族学院环境与资源学院,大连116600

出　　处：《分析化学》2014年第10期1535-1538,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.30872004);中央高校基本科研业务费专项(Nos.DC12010305;DC 12010204)资助~~

摘　　要：采用微透析法获取3种杨树嫩茎质外体汁液的灌流液,流速1μL/min,探针截留分子量为30 kDa,无须处理直接通过原子吸收光谱(石墨炉法)测定样品中Na+,K+,Ca2+浓度,通过建立实时、无损检测离子浓度变化的实验技术,进行杨树生理生化的研究。此方法加标回收率在95.8%~103.1%之间。研究表明,吴屯杨、小胡杨和小美旱的Na+含量分别为1034~1156μg/L,1493~1611μg/L和1586~1703μg/L;K+含量分别为1012~1237μg/L,941~964μg/L和1095~1201μg/L;Ca2+含量分别为4976~5237μg/L,4786~5042μg/L和5893~6142μg/L,本方法为在植物应激反应中通过离子浓度变化来适应外界环境的研究提供可靠数据。The dialysis for three species of poplar stems apoplast was obtained by microdialysis method, and then the concentration of Na＋, K＋, Ca2＋ in the dialysis sample was directly measured by graphite furnace atomic absorption spectrometry （ GF-AAS） . The flow rate of perfusate was 1μL/min and the cut-off molecular weight of probe was 30 kDa. The real-time and non-destructive test technique established for the detection of ion concentration could be applied in research of poplar biochemical physiology. The recovery of the proposed method was 95. 8%-103. 1%. The contents of sodium ion in Populus wutunensis, Populus simonii and Salix matsudana were 1034-1156 μg/L, 1493-1611 μg/L and 1586-1703 μg/L; potassium ion were 1012-1237 μg/L, 941-964 μg/L and 1095-1201 μg/L; calcium ion were 4976-5237 μg/L, 4786-5042 μg/L and 5893-6142 μg/L. The method could provide reliable data for the study of ion concentration changes of plant response to adapt to all kinds of external environment.

关 键 词：杨树 质外体 微透析 金属元素 

分 类 号：S792.11[农业科学—林木遗传育种]