基于曲线拟合的蚕豆病害叶的FTIR研究  

Curve-fitting Based FTIR of Diseased Leaves in Broad Bean

作　　者：汪小华[1] 刘刚[1] 欧全宏[1] 周湘萍[1] 郝建明[1] 刘剑虹[2] 汪禄祥[3] 

机构地区：[1]云南师范大学物理与电子信息学院,昆明650500 [2]云南师范大学可再生能源材料先进技术与制备教育部重点实验室,昆明650500 [3]云南省农业科学院质量标准与检测技术研究所,昆明650223

出　　处：《湖北农业科学》2014年第17期4182-4185,共4页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(30960179)

摘　　要：用傅里叶变换红外光谱法研究蚕豆病害叶片,结果显示不同病害蚕豆叶片红外光谱图整体相似,它们的红外光谱主要由蛋白质、脂类和多糖的振动吸收带组成,仅在1 800～1 300 cm-范围光谱的峰位、峰形及吸收强度有一些微小差异.对1 800～1 300 cm-1波数范围的光谱图进行二阶导数处理,结果显示蚕豆病害叶的二阶导数谱差异明显.对健康和病害蚕豆叶1 700～1 500 cm-1范围光谱进行傅里叶自去卷积和曲线拟合处理后,得到蛋白质酰胺Ⅱ带（1 550 cm-1）、木质素（1 605 cm-1）和酰胺Ⅰ（1 650 cm-1）3个子峰,相应子峰的峰面积比例显示差异,黄化卷叶病分别为24.01％、36.55％、39.44％,赤斑病分别为15.42％、42.98％、41.61％,轮纹病分别为32.39％、35.63％、31.98％,锈病分别为13.97％、46.40％、39.65％,健康叶片分别为38.86％、28.68％、32.47％,健康叶的酰胺Ⅱ带子峰相对面积比病害叶的大,而其木质素子峰相对面积比病害叶的小.对于子峰面积比A1563A1605、A1650/A 1605和A1563/A1654,4种病害叶的比值均比健康叶的相应数值小,4种病害叶之间也有差异.结果表明傅里叶变换红外光谱（FTIR）结合曲线拟合可望对不同病害的样品进行有效鉴别.Fourier transform infrared （FTIR） method was used to study diseased leaves in broad bean. Results showed that the infrared spectra of broad bean leaves were similar and mainly made up of the absorption bands of protein, lipid and polysaccharides. There were minor differences including the spectral peak position, peak shape and the absorption intensity in the range of 1 800-1 300 cm-1. There were obvious differences among their second derivative spectra in the range of 1 800- 1 300 cm-1. After the procedure of the Fourier self-deconvolution and curve fitting of health bean leaf and broad bean diseased leaves in the range of 1 700-1 500 cm-1, three peaks were obtained at 1 560 cm-1 （amide Ⅱ ）, 1 605 cm-1 （lignin） and 1 650 cm-1 （protein amide Ⅰ）. The ratios of relative areas of the bands of amide Ⅱ , lignin, and amide Ⅰ were 38.86%, 28.68% and 32.47% in the spectrum of healthy leaf, respectively. It was distinguished from the diseased leaves （red spot leaf： 15.42%, 42.98% and 41.61%, ring spot leaf： 32.39%, 35.63% and 31.98%, rust leaf： 13.94%, 46.40% and 39.65%, yellowing leaf curl disease leaf： 24.01%, 36.55% and 39.44%）. For sub-band area ratios A1563/A1605, A1650/A1605 and A1563/A1654, those of four kinds of diseased leaves were smaller than that of healthy leaf. The results proved that FFIR combined with curve fitting might be a potentially useful tool for detecting different kinds of broad bean diseases.

关 键 词：傅里叶变换红外光谱 蚕豆病害 二阶导数光谱 曲线拟合 

分 类 号：O657.33[理学—分析化学] S435.23[理学—化学]