苏云金芽胞杆菌BRC-ZLL5类细菌素的抑菌谱和理化特性  

Antibacterial spectrum and physiochemical property of bacteriocin-like inhibitory substances from Bacillus thuringiensis BRC-ZLL5

作　　者：黄天培[1] 吴小凤[2] 潘洁茹[3] 蒋捷[1] 肖颖[1] 张灵玲[1] 关雄[1] 

机构地区：[1]福建农林大学生物农药与化学生物学教育部重点实验室,福州350002 [2]泉州市疾病预防控制中心,泉州362018 [3]福州市疾病预防控制中心,福州350004

出　　处：《应用与环境生物学报》2014年第5期779-784,共6页Chinese Journal of Applied and Environmental Biology

基　　金：“十二五”农村领域国家科技计划课题(2011AA10A203);国家自然科学基金项目(31201574,31071745);福建省教育厅高校领军人才项目(k8012012a)资助~~

摘　　要：为提升苏云金芽胞杆菌(Bacillus thuringiensis,简称Bt)生物农药产品附加值,通过对其发酵上清液提取细菌素,进一步研究Bt菌株BRC-ZLL5类细菌素(Bacteriocin-like inhibitory substa nces,BLIS)的抑菌谱和理化特性.结果显示:BRC-ZLL5类细菌素对李斯特菌、铜绿假单胞杆菌、粪链球菌具有活性,对4种肠道益生菌及自身无抗菌活性;抑菌活性动态监测表明其活性集中于6-18 h,在12 h达到最大值;分子量(Mr)小于1.43×104;具有较广的pH耐受范围(pH 3-9)和较强的耐热能力(50-90℃时活性极显著提高;121℃高压灭菌15 min仍保持32%活性);脂肪酶Ⅱ可极显著提高其活性(P<0.01),蛋白酶K、α胰凝乳蛋白酶Ⅶ、α胰凝乳蛋白酶Ⅱ使其活性消失(P<0.01).BRC-ZLL5类细菌素可望用于进一步开发,本研究为高效利用Bt发酵上清液提供了依据.To add value to Bacillus thuringiensis（Bt） products, extraction of bacteriocins from Bt fermentation supernatants is needed. This paper aimed to study the extraction methods and the property of Bt bacteriocin-like inhibitory substances（BLIS）. In this study, antibacterial spectrum and physiochemical property of Bt BRC-ZLL5 BLIS were investigated using Listeria monocytogenes（Lm） as one of the indicator strains. The results showed that BRC-ZLL5 BLIS were active against Listeria, Pseudomonas aeruginosa and Streptococcus faecalis, but not against itself and 4 probiotics. The activity of BRC-ZLL5 BLIS against Lm 100525 existed for 6-18 h after incubation, peaking at 12 h after incubation. The molecular weight（Mr） of BRCZLL5 BLIS was less than 1.43 × 104. The BRC-ZLL5 BLIS were pH stable（pH 3-9） and heat-resistant： compared with that at 4 °C, the activity of BRC-ZLL5 BLIS was significantly increased after treatment at 50-90 °C for 30 min; it retained 32% activity after treatment at 121 °C for 15 min. The antibacterial activity of BRC-ZLL5 BLIS was increased with addition of lipase II（P 0.01） while completely lost after treatment with proteinase K, α-chymotrypsin VII and α-chymotrypsin II（P 0.01）. This study provides a model for utilization of Bt fermentation supernatants with high efficiency.

关 键 词：细菌素 苏云金芽胞杆菌 抑菌谱 理化特性 

分 类 号：Q936[生物学—微生物学]