宫颈上皮病变组织中MEKK3、NF-κBmRNA的表达及意义  

The expression of MEKK3 and NF-κB mRNA in cervical intraepithelial lesions and its significance

作　　者：曹学全[1] 章辉[1] 范广民[1] 卢洪胜[1] 杨朝晖[1] 甘梅富[2] 顾华敏[1] 

机构地区：[1]浙江省台州市中心医院病理科,318000 [2]台州医院病理科

出　　处：《中华全科医学》2014年第12期1923-1925,1937,F0003,共5页Chinese Journal of General Practice

摘　　要：目的探讨有丝分裂原活化蛋白激酶激酶激酶(MEKK3)、核转录因子κB(NF-κB)mRNA在宫颈上皮病变组织中表达及其与宫颈癌临床病理因素的关系,进一步分析两者在宫颈癌中表达的相关性。方法应用原位杂交方法检测90例宫颈癌、76例宫颈上皮内瘤变(CIN)及30例慢性宫颈炎组织中MEKK3、NF-κB mRNA表达情况,结合患者的临床病理资料进行统计学分析。结果在30例慢性宫颈炎,76例宫颈CIN,80例宫颈鳞癌中,MEKK3 mRNA阳性表达率的阳性率分别为13.3%(4/30)、51.3%(39/76)、82.5%(66/80),组间差异均有统计学意义(P<0.05)。NF-κB mRNA在宫颈鳞癌中阳性表达率为73.8%(59/80),均高于宫颈CIN的40.8%(31/76)和慢性宫颈炎的10.0%(3/30),差异均有统计学意义(P<0.05)。宫颈CINⅢ中MEKK3、NF-κB mRNA阳性表达率均高于宫颈CINⅡ和CINⅠ,差异均有统计学意义(P<0.05),而宫颈CINⅡ中MEKK3、NF-κB mRNA阳性表达率略高于宫颈CINⅠ,差异无统计学意义(P>0.05)。MEKK3、NF-κB mRNA阳性表达与宫颈癌患者临床分期、浸润深度和是否发生淋巴结转移间的差异均具有统计学意义(P<0.05)。宫颈癌中MEKK3 mRNA和NF-κB mRNA的表达呈正相关(P<0.05)。结论宫颈癌组织中MEKK3、NF-κB mRNA呈现高表达,MEKK3、NF-κB mRNA表达与宫颈癌的发生、浸润、转移和预后密切相关,两者可能在宫颈癌的发生过程中起着协同调控作用。Objective To investigate the expression of mitogen-actived protein kinase/extracellular signal-regulated kinase kinase kinase 3 （MEKK3） and nuclear factor-κB （NF-κB） mRNA in cervical intraepithelial lesions and its relationship with clinicopathological factors of cervical cancer, analyzing correlation between the expression of MEKK3 and NF-κB mRNA in cervical carcinoma furtherly. Methods The expression of MEKK3 and NF-κB mRNA was detected in 90 cases of cervical carcinoma,76 cases of cervical intraepithelial neoplasia（CIN） and 30 cases of chronic cervicitis by in situ hy- bridization method, the clinical and pathological data of patients were statistically analyzed. Results In 30 cases of chro- nic cervicitis,76 cases of cervical CIN,80 cases of cervical squamous cell carcinoma,the positive rates of MEKK3 mRNA were 13.3 % （4/30） ,51.3 % （ 39/76 ）, 82.5 % （ 66/80 ） respectively, there was significant difference among them （ P 〈 0.05 ）. The positive rate of NF-KB mRNA in cervical carcinoma was 73.8 % （ 59/80 ）, higher than that in CIN （40.8 % ） and in chronic cervieitis（ 10% ）,there were significant differences among them（P 〈 O. 05 ）. The positive expression of MEKK3, NF-KB mRNA were slightly higher than those in cervical CIN I and CIN Ⅱ , there wasnot significant difference between them（P 〉 0.05 ）. The positive rates of MEKK3mRNA and NF-KB mRNA were correlated with in clinical staging, infiltration depth and lymphode metastasis of cervical carcinoma respectively（ P 〈 0.05 ）. The expression of MEKK3 mRNA and was positively correlated with the expression of NF-κB mRNA in cervical carcinoma（ P 〈0. 05 ）. Conclusion MEKK3mRNA and NF-KBmRNA in cervical carcinoma tissues showed overexpression, the expressions of MEKK3 and NF-κBmRNA were closely correlated with occurrence, invasion, metastasis and prognosis of cervical carcinoma, which of both may play synergistic regulation in the course of occurrence of cervical carcinoma.

关 键 词：有丝分裂原活化蛋白激酶激酶激酶 核转录因子ΚB 原位杂交 宫颈癌 

分 类 号：R737.33[医药卫生—肿瘤] R365[医药卫生—临床医学]