乙型肝炎病毒在非霍奇金淋巴瘤肿瘤组织中的表达及意义  

Detection of HBV at non-hodgkin's lymphoma organizations and its significance

作　　者：吴遐[1] 王俊和[2] 郝磊[1] 李程[1] 魏敏[1] 邓芳[1] 

机构地区：[1]安徽省肿瘤医院检验科,合肥市230031 [2]安徽省蚌埠医学院第一附属医院血液科,233004

出　　处：《中华全科医学》2014年第12期1938-1940,共3页Chinese Journal of General Practice

摘　　要：目的探讨血清HBsAg和HBcAb阳性的非霍奇金淋巴瘤(NHL)患者骨髓或淋巴结乙型肝炎病毒(HBV)表达水平,乙型肝炎病毒是否侵犯其骨髓及淋巴组织。方法将血清HBsAg和HBcAb阳性的骨髓或淋巴结标本分4组:133例NHL新鲜骨髓标本;233例NHL相应新鲜淋巴结活检标本;315例NHL淋巴结蜡块标本;412例缺铁性贫血患者新鲜骨髓标本作为对照组。应用免疫组化法检测骨髓和淋巴标本的乙型肝炎病毒表达水平。结果乙型肝炎病毒在NHL的新鲜骨髓、NHL的新鲜淋巴结、NHL的淋巴结蜡块及对照组缺铁性贫血患者新鲜骨髓标本中的阳性表达率,分别为69.7%(23/33)、12.1%(4/33)、0%(0/15)和0%(0/12)。乙型肝炎病毒在NHL新鲜骨髓组织表达水平高于NHL的新鲜淋巴结、NHL的淋巴结蜡块及对照组缺铁性贫血患者新鲜骨髓标本,差异有统计学意义(χ2=22.627,P<0.000;χ2=20.073,P<0.000;χ2=17.107,P<0.000)。乙型肝炎病毒在NHL的新鲜淋巴结与淋巴结蜡块组织表达水平相比较,差异无统计学意义(χ2=0.714,P=0.398>0.05)。结论在血清HBsAg和HBcAb阳性的非霍奇金淋巴瘤患者乙型肝炎病毒在骨髓组织表达水平高于淋巴结组织,乙型肝炎病毒主要侵犯骨髓组织,淋巴组织侵犯较少。Abstract：Objective To investigated the expression level of hepatitis B virus（HBV） in bone marrow or lymph node tissues of the serum HBsAg and HBcAb positive in Non-Hodgkin＇ s Lymphoma（NHL） patients,hepatitis B virus was a violation of the bone marrow and lymph node tissues. Methods The serum HBsAg and HBcAb positive bone marrow or lymph node specimens were divided into 4 groups ：@33 cases of NHL fresh bone marrow specimens ;（1）33 cases of NHL corresponding fresh lymph node biopsy specimens;（1）15 cases of NHL lymph node paraffin embedded specimens;（2）12 cases of patients with iron deficiency anemia fresh bone marrow specimens as control group. The immunohistochemistry was used to detect the hepatitis B virus expression in each sample of bone marrow and lymph node. Results The positive ex- pression of hepatitis B virus was detected in NHL fresh bone marrow group, NHL fresh lymph node group, NHL lymph node paraffin embedded group and control group with iron deficiency anemia fresh bone marrow, respectively 69.7% （23/ 33）, 12.1% （4/33） ,0% （0/15） and 0% （0/12）. Hepatitis B virus expression level in NHL fresh bone marrow group was higher than that of NHL fresh lymph node group, NHL lymph node paraffin embedded group and control group with i- ron deficiency anemia fresh bone marrow, the difference was statistically significant （χ2 = 22. 627, P 〈 0. 000 ; χ2 = 20. 073 ,P 〈0.000;χ = 17. 107 ,P 〈 0. 000）. Hepatitis B virus expression level between NHL fresh lymph node group and NHL lymph node paraffin embedded group was not statistically significant（χ2 = 0. 714 ,P = 0. 398 〉 0.05 ）. Conclu- sion Hepatitis B virus（HBV） expression level in bone marrow is higher than that of lymph node tissues of the serum HBsAg and HBcAb positive in Non-Hodgkin＇ s Lyrnphoma（NHL） patients ,hepatitis B virus mainly affects the bone marrow, lymph node invasion less.

关 键 词：乙型肝炎病毒 非霍奇金淋巴瘤 骨髓 淋巴组织 

分 类 号：R733.1[医药卫生—肿瘤]