A-NK细胞对舌鳞癌裸鼠皮下移植瘤抑制作用的实验研究  

The effects of A-NK cells on tumor growth of human tongue squamous cell carcinoma in nude mice

作　　者：祝军[1] 刘红[1] 张真[1] 刁晓洁[1] 

机构地区：[1]新疆医科大学附属中医医院口腔科,乌鲁木齐830000

出　　处：《实用口腔医学杂志》2014年第6期753-756,共4页Journal of Practical Stomatology

基　　金：自治区科技支疆项目(编号:201191158)

摘　　要：目的：探讨A-NK细胞治疗裸鼠舌鳞癌移植瘤的效果。方法：分离培养人A-NK细胞;建立舌鳞癌Tca8113-Tb细胞皮下移植瘤模型,随机分为2组（n=7）对照组裸鼠瘤旁注射生理盐水,实验组裸鼠瘤旁注射A-NK细胞,观察33 d后,处死裸鼠,剥离移植瘤,称瘤重,绘制肿瘤生长曲线。结果：治疗15-33 d时实验组肿瘤体积均小于对照组（P〈0.05）,观察33 d后,实验组和对照组瘤重（g）分别是为0.96±0.38和3.74±1.22（P〈0.05）。结论：A-NK细胞在裸鼠体内具有抗肿瘤效应。Objective： To explore the treatment effect of A-NK cells on the subcutaneously transplanted tumor of tongue squamous cell carcinoma in nude mice. Methods： Tca8113-Tb cells were subcutaneously transplanted into nude mice,14 tumor bearing mice were randomly assigned to 2 groups（n=7）. The mice in control group were injected with normal saline,those in the experimental group were injected with A-NK cells. All animals were killed 33 days after tumor cell transplantation,The tumor volume and weight of the mice were measured and compared. Results： A-NK cells significantly inhibited tumor growth in nude mice in terms of the volume（P〈0. 05）. After 15- 33 d of treatment the tumor weight （g） in the treated and control mice were 0. 96 ± 0. 38 and 3. 74 ± 1. 22 respectively（P〈0. 05）. Conclusion： A-NK cells can inhibit the growth of Tca8113-Tb cell induced tumor in nude mice.

关 键 词：A-NK细胞 舌鳞状细胞癌 裸鼠移植瘤 抗肿瘤效应 

分 类 号：R739.8[医药卫生—肿瘤]