肝细胞生长因子对人舌鳞癌Tca8113细胞VEGF-C表达的影响及作用机制的初步研究  

The influence of HGF on the expression of VEGF-C and its mechanism in human tongue squamous cell carcinoma Tca8113 cells

作　　者：杜洪亮[1] 何等旗[1] 张志瑞[1] 徐继莲 席亚明[1] 

机构地区：[1]兰州大学第一医院口腔颌面外科,730000

出　　处：《实用口腔医学杂志》2014年第6期792-795,共4页Journal of Practical Stomatology

基　　金：甘肃卫生行业科研计划项目(编号:GSWST2010-04)

摘　　要：目的:研究HGF对人舌鳞癌Tca8113细胞中VEGF-C表达的影响并探讨其作用机制。方法:体外培养Tca8113细胞,应用ELISA方法测定不同浓度HGF作用下以及分别用LY294002、U0126、SP600125、SB203580信号通路抑制剂阻断PI3K/Akt、P44/P22MAPK、JNK、P38MAPK信号通路后VEGF-C的表达水平。结果:随着培养液中HGF浓度的增加,Tca8113细胞中VEGF-C的表达水平出现先增高后降低的趋势,当HGF浓度为40 ng/ml时,VEGF-C表达水平最高。PI3K/Akt信号通路抑制剂(LY294002)和P42/44MAPK信号通路抑制剂(U0126)显著抑制了HGF刺激下Tca8113的VEGF-C蛋白的表达(P<0.01);而JNK信号通路抑制剂(SP600125)和P38MAPK信号通路抑制剂(SB203580)对HGF刺激下Tca8113的VEGFC蛋白的表达影响甚微(P>0.05)。结论:在人舌鳞癌Tca8113细胞中,随着HGF浓度的增加,VEGF-C的表达先增高后降低。PI3K/Akt和P44/P22MAPK信号通路在口腔鳞状细胞癌淋巴转移中可能发挥作用。Objective： To investigate the influence of hepatocyte growth factor（HGF） on the expression of vascular endothelial growth factor C（VEGF-C） and the mechanism of HGF-induced VEGF-C expression in tongue squamous cell carcinoma Tca8113 cells. Methods： Tca8113 cells were cultured and exposed to HGF with various concentrations. The expression level of VEGF-C was assessed by ELISA. Signaling transduction inhibitors LY294002,U0126,SP600125,SB203580 was used to block PI3 K/Akt,P44 /P22 MAPK,JNK,P38 MAPK signaling pathways,respectively. Then,the expression level of VEGF-C was detected by ELISA. Results： The VEGF-C expression of Tca8113 cells increased at the beginning and decreased later with the increase of HGF concentration. When the concentration of HGF was 40 ng/ml,VEGF-C expression level was the highest. Inhibitor LY294002 of PI3 K/Akt and Inhibitor U0126 of P44/P22 MAPK significantly blocked the effects on HGF-induced VEGF-C up-regulation（P〈0. 01）. Inhibitor SP600125 of JNK and inhibitor SB203580 of P38 MAPK didn＇ t interfere HGF-induced VEGF-C expression（P〈0. 05）. Conclusion：HGF contributed to the expression of VEGF-C,PI3 K/Akt and P44/P22 MAPK signaling pathways may be involved in HGF-induced VEGF-C up-regulation,and may play potential roles in lymphatic metastasis of oral squamous cell carcinoma.

关 键 词：口腔鳞状细胞癌 肝细胞生长因子 血管内皮生长因子C 信号通路 信号通路抑制剂 

分 类 号：R739.8[医药卫生—肿瘤]