siRNA干扰Mcl-1对唾液腺腺样囊性癌SACC-2细胞生物活性的影响  

The effects of siRNA targeting Mcl-1 on biological behavior of salivary adenoid cystic carcinoma SACC-2 cells

作　　者：张瑞智[1] 张萍[1] 余波[1] 罗锐[1] 龚正林[1] 

机构地区：[1]安康市中心医院口腔科,725000

出　　处：《实用口腔医学杂志》2014年第6期809-812,共4页Journal of Practical Stomatology

摘　　要：目的:探讨siRNA干扰髓样细胞白血病-1分子(Mcl-1)对唾液腺腺样囊性癌细胞生物活性的影响。方法:构建Mcl-1-siRNA,转染入SACC-2细胞。应用real-time PCR法检测Mcl-1 mRNA表达水平及Western blotting检测Mcl-1蛋白表达水平。分别采用MTT法、Transwell小室法、流式细胞法观察Mcl-1对SACC-2细胞增殖、迁移及凋亡能力的影响。结果:与空白对照组、脂质体组和NC-siRNA组相比,Mcl-1-siRNA组SACC-2细胞的增殖速度明显减缓;转染48 h后,Mcl-1-siRNA组SACC-2细胞迁移数为(39±9.0)个,明显低于对照组(69±6.0)个;Mcl-1-siRNA组SACC-2细胞凋亡细胞百分率为8.6%(对照组为1.9%)。结论:沉默Mcl-1显著抑制唾液腺腺样囊性癌SACC-2细胞增殖和迁移能力,促进其凋亡。Objective： To explore the effect of siRNA targeting myeloid cell leukemia-1（Mcl-1） on the biological behavior of salivary adenoid cystic carcinoma cells. Methods： The chemically synthesized Mcl-1-siRNA was transfected into salivary adenoid cystic carcinoma SACC-2 cells. The expression levels of Mcl-1-mRNA and Mcl-1 protein were examined by Real-time PCR and western blotting respectively. MTT assay,transwell chamber and flow cytometry were used to determine the effect of Mcl-1-siRNA on SACC-2 cell proliferation,migration and apoptosis. Results： Compared with the control group,liposome group and NC-siRNA group,SACC-2 cell proliferation rate of Mcl-1-siRNA group was obviously slowed down. 48 h after transfection,the migration of SACC-2 cells in Mcl-1-siRNA group（39 ± 9. 0） were lower than that in control group（69 ± 6. 0）. The apoptosis rate of Mcl-1-siRNA group（8. 6%） was significantly higher than that in control group（1. 9%）. Conclusion： Silence Mcl-1 can inhibit cell proliferation and migration and promote apoptosis of salivary adenoid cystic carcinoma cells.

关 键 词：MCL-1 唾液腺腺样囊性癌细胞 增殖 迁移 凋亡 

分 类 号：R739.8[医药卫生—肿瘤]