姜黄素对Jurkat细胞增殖及凋亡的影响  

Effect of curcumin on proliferation and apoptosis of Jurkat cells

作　　者：丁浩然[1] 金焱[1] 浦长春 于忠臣[1] 韩立杰[1] 王弘珺[1] 

机构地区：[1]吉林医药学院,吉林吉林132013

出　　处：《中国民康医学》2014年第21期1-2,5,共3页Medical Journal of Chinese People’s Health

基　　金：吉林省科技厅资助项目(20130101140JC);吉林省大学生创新创业训练计划项目(2013)

摘　　要：目的:探讨姜黄素对Jurkat细胞增殖及凋亡的影响。方法:体外培养条件下,用姜黄素处理Jurkat细胞12h后,MTT法检测细胞的生存率和IC50值;Hoechst33342染色观察细胞凋亡的形态变化;流式细胞学检测细胞凋亡率。结果:体外培养条件下,姜黄素对Jurkat细胞的增殖有明显抑制作用,呈剂量依赖性,IC50值为31μmol/L。激光共聚焦扫描显微镜观察,随着姜黄素浓度的增加,Jurkat细胞凋亡增多。流式细胞学分析表明,低浓度姜黄素使Jurkat细胞发生早期凋亡,高浓度姜黄素使Jurkat细胞出现晚期凋亡。结论:姜黄素明显抑制Jurkat细胞增殖,同时诱导Jurkat细胞发生凋亡。Objective：To investigate effect of curcumin on proliferation and apoptosis of Jurkat cells. Methods：Under in vitro conditions, Jurkat cells were treated with different concentrations of curcumin for 12h. The cells were harvested for MTT analysis （sur-vival rate and IC50）. Meanwhile, the apoptosis cells were determined with Hoechst33342 staining for morphologic changes and flow cytometry for apoptotic rate. Results： The proliferation of Jurkat cells was obviously inhibited in a dose-dependent manner （ IC50=31μmol/L） by curcumin under the in vitro conditions. The number of apoptosis cells increased with the concentration of curcumin un-der confocal laser microscope. After analyzed with flow cytometry, it found that early apoptosis cells were more in lower curcumin con-centration, and late apoptosis cells were much more in higher curcumin concentration. Conclusions：Curcumin inhibits the proliferation of Jurkat cells and induces apoptosis of Jurkat cells.

关 键 词：姜黄素 细胞增殖 凋亡 

分 类 号：R733.7[医药卫生—肿瘤]