HPLC法测定淀粉中马来酸的不确定度评定  

Uncertainty Evaluationfor the Determination of Maleic Acid in Starch by High Performance Liquid Chromatography

作　　者：烟利亚[1] 

机构地区：[1]上海天祥质量技术服务有限公司,上海200233

出　　处：《广州化工》2014年第20期145-148,共4页GuangZhou Chemical Industry

摘　　要：建立了HPLC测定淀粉中马来酸的分析方法,并进行了不确定度评定。根据CNAS-GL06-2006《化学分析中不确定度的评估指南》和JJF1059.1-2012《测量不确定度评定与表示》规定的原理与方法,对HPLC测定淀粉中马来酸含量测量不确定性的来源进行分析,建立了测量不确定度评定的数学模型,计算并评定了测量结果的合成不确定度。结果表明:以HPLC测定淀粉中马来酸含量,其不确定度主要来源于校准曲线拟合和马来酸标准溶液,其次为测量重复性和高效液相色谱仪。A High Performance Liquid Chromatography （ HPLC） method was set up to detect maleic acid in starch , and the measurement uncertainty of this method was evaluated.According to the principle and method in CNAS -GL06-2006 Guidance on Evaluating the Uncertainty in Chemical Analysis and JJF1059.1-2012 Evaluation and Expression of Uncertainty in Measurement, the components of uncertainty in the measuring process were analyzed , a mathematic model was established , and the combined uncertainty was calculated and evaluated.The results showed that the major factors of uncertainty in measurement were the establishment of the standard curve and the preparation of standard solution , and the minor ones were measurement repeatability and HPLC.

关 键 词：高效液相色谱法 淀粉 马来酸 不确定度 

分 类 号：O657.72[理学—分析化学]