CALCA基因遗传变异及其与肉质性状关联分析  

Analysis on Polymorphism of CALCA Gene in Zaosheng Cattle and Zaosheng Hybrids Cattle and Their Association with Carcass Trait

作　　者：张彩霞[1] 赵生国[1] 李天科[1] 雷赵民[1] 

机构地区：[1]甘肃农业大学动物科学技术学院,甘肃兰州730030

出　　处：《华北农学报》2014年第5期114-118,共5页Acta Agriculturae Boreali-Sinica

基　　金：甘肃省农业生物技术研究与应用开发项目(GNSW-2010-04;GNSW-2011-27);甘肃省农业科技创新项目;甘肃省高等学校科研项目(2013A-069);兰州市科技局玉米秸秆饲料化关键技术集成与示范推广项目(2012-2159);甘肃省农牧厅生物技术专项(CNSW-2010-05;CNSW-2012-25)

摘　　要：旨在分析早胜牛及其杂交牛CALCA基因第4外显子的多态性及其与肉质性状的相关性,发现与肉质性状相关的分子标记,为加快肉牛选育进程提供参考。利用PCR-SSCP技术对早胜牛、南杂牛、秦杂牛和西杂牛共362个个体的CALCA基因第4外显子进行了遗传变异检测,并运用SPSS 17.0软件对早胜牛(平凉类群)遗传变异与经济性状关联性进行了分析。结果表明,CALCA基因第4外显子存在2个突变位点T3237A和G3289A,并检测到AA和AB2种基因型。相关性分析表明,CALCA基因突变位点与剪切力、眼肌面积、热胴体重和熟肉率显著相关(P<0.05),AA基因型个体剪切力、热胴体重和熟肉率显著高于AB基因型个体(P<0.05),而AB基因型个体眼肌面积显著高于AA基因型个体(P<0.05),其他性状在各基因型间差异不显著。因此,初步推测CALCA基因的突变位点可以作为评定早胜牛肉质性状的遗传标记。The aim was to analyze association of CALCA gene polymorphisms with some economic traits in Zaosheng cattle and Zaosheng crossbred,and to evaluate it as a candidate molecular marker associated with beef quality. PCR-SSCP method was used to detect the genetic variation in CALCA gene in 362 cattle from 5 cattle groups,including Zaosheng in Qingyang Prefecture,Zaosheng cattle in Pingliang Prefecture,Nanza（ South Devon cattle × Qingyang groups）,Qinza（ Qinchuan cattle × Pingliang groups）,Xiza（ Simmental × Pingliang groups）. The association between SNP locus and some carcass traits of Pingliang groups were analyzed. Two genotypes AA and AB were detected at exon 4 in CALCA gene. Analysis of association of polymorphism with carcass traits at mutation locus showed that there were significant effects on WBSF,Ribeye area,Hot carcass weight and Cooking loss（ P 〈0. 05）and there was no effect on other traits（ P 〉0. 05）. In conclusion,SNP of CALCA gene could be a genetic mark for carcass trait.

关 键 词：肉牛 CALCA基因 SNP 群体遗传分析 肉质性状 

分 类 号：S823[农业科学—畜牧学]