去铁胺对人源肠道Caco-2细胞成纤维细胞生长因子21表达的影响  

Effect of hypoxia-mimic deferoxamine on fibroblast growth factor 21 expression in intestine cells

作　　者：何雪倩 张翼[1] 吴玲剑[2] 陈超[1] 谢尧琪 陈李斌佶[1] 刘彦隆[1] 林虹[1] 庞玲霞[1] 

机构地区：[1]温州医科大学,浙江温州325035 [2]温州医科大学附属第一医院,浙江温州325035

出　　处：《山东医药》2014年第40期1-3,共3页Shandong Medical Journal

基　　金：浙江省自然科学基金项目(LY12H03001;LQ13H280002);浙江省新苗人才计划项目(2013R413021);温州医科大学学生科研立项资助课题(wyx201201039)

摘　　要：目的观察缺氧模拟物去铁胺(DFO)对人源肠道Caco-2细胞成纤维细胞生长因子21(FGF21)表达的影响。方法分别用50、100、150、200μmol/L的DFO处理Caco-2细胞12 h,同时用200μmol/L的DFO处理Caco-2细胞4、8、12 h,RT-PCR法检测细胞中的FGF21 mRNA。分别用缺氧诱导因子(HIF)抑制剂和ROS抑制剂预处理Caco-2细胞,再用DFO继续处理,RT-PCR法检测细胞中的FGF-21 mRNA。结果随DFO浓度增加和作用时间延长,Caco-2细胞FGF21 mRNA相对表达量逐渐降低(P均<0.05)。HIF抑制剂预处理后,Caco-2细胞FGF21相对表达量依然降低(P均<0.05);ROS抑制剂预处理后,Caco-2细胞FGF21 mRNA相对表达量未出现明显降低(P均>0.05)。结论 DFO对肠道Caco-2细胞FGF21的表达有抑制作用,该作用与缺氧产生HIF无关,而与ROS有关。Objective To study the effect of hypoxia-mimic deferoxamine（ DFO） on fibroblast growth factor（FGF21） expression in intestinal Caco-2 cells. Methods Caco-2 cells were either treated with 50,100,150,200 μmol/L DFO for 12 h or treated with 200 μmol /L DFO for 4,8,12 h. FGF21 mRNA expression were measured by RT-PCR.Caco-2 cells were pretreated with either HIF or ROS inhibitor and then treated with DFO. FGF21 mRNA expression was measured by RT-PCR. Results FGF21 mRNA expression decreased in dose-and time-dependent manners with DFO treatment in Caco-2 cells. HIF inhibitor and DFO treatment still decreased FGF21 mRNA expression（all P〈0. 05）. But ROS inhibitor pretreatment reversed DFO-induced FGF21 mRNA down-regulation（ all P〉0. 05）. Conclusion DFO-induced FGF21 down-regulation is not associated with HIF stabilization,but involves hypoxia-induced oxidative stress.

关 键 词：缺氧 去铁胺 CACO-2细胞 成纤维细胞生长因子 

分 类 号：R363[医药卫生—病理学]