外源诱抗剂对烟草青枯病的诱抗效果研究  

Inhibition Effects of Induced Resistance on Tobacco Resistance to Ralstonia solanacearum

作　　者：江厚龙[1,2] 李鹏[3] 李钠钾[1,2] 催伟伟 张艳[1,2] 耿丽娜[1,2] 丁伟[1,2] 

机构地区：[1]重庆烟草科学研究所 [2]西南大学植物保护学院/植物保护博士后科研流动站,重庆400715 [3]平顶山学院,河南平顶山467000

出　　处：《中国农学通报》2014年第28期286-290,共5页Chinese Agricultural Science Bulletin

基　　金：重庆市烟草专卖局项目"重庆烟区不适用鲜烟叶优化技术研究"(NY20130501070003);重庆市烟草专卖局项目"重庆烟草壮苗培育技术研究与示范(NY20120301070011)

摘　　要：为研究外源诱抗剂对烟草青枯病的诱抗效果,利用外源水杨酸、核黄素和草酸等诱抗物质处理烟苗,研究3种物质处理后接种青枯病对相关酶活性、可溶性蛋白含量、病情指数和相对防效的影响。结果表明,3种诱抗物质均能提高叶片内的苯丙氨酸酶(PAL)、过氧化物酶(POD)、多酚氧化酶(PPO)的活性及可溶性蛋白含量,但却降低了过氧化氢酶(CAT)活性。3种物质均可诱导烟草对青枯病的抗性,前期以核黄素的诱抗效果较好,平均防效达55.71%(5天),且与水杨酸差异不显著;中期(8天)以水杨酸防效较好,防效为42.20%,显著高于其他2种物质;后期水杨酸的诱抗效果好,平均防效达33.67%(11天)。The objectives of this study were to investigate the inhibition effects of induced resistance on tobacco resistance to Ralstonia solanacearum. The effects of disease index, relative control effect, enzyme activities and content of water-soluble protein were studied after the tobacco leaves had been treated with salicylic acid（SA）,riboflavin and oxalate acid, and inoculated with Ralstonia solanacearum the next day. The results showed that after leaf treated with the three induced-resistances, the activities of PAL, POD and PPO, the content of soluble protein were increased while CAT activities were inhibited in leaves. The three resistant inducers couldinduce the tobacco to produce resistance to Ralstonia solanacearum. At earlier stage（5 d）, the riboflavin hadthe best induced resistance effects, the effective percentage was 55.71%, and there were no significant differences with SA. At mid-term（8 d）, SA had the highest induced resistance effects, the effective percentagewas 42.20%, and significantly higher than the other two. At later stage（11 d）, SA had the best induce dresistance effects; the effective percentage was 33.67%.

关 键 词：烟草 水杨酸 核黄素 草酸 诱导抗性 

分 类 号：S572[农业科学—烟草工业]