高效液相色谱法测定食用植物油中游离棉酚含量方法的建立  

Establishment of method for determination of free gossypol content in edible vegetable oil by high performance liquid chromatography

作　　者：刘扬[1] 徐晓枫[2] 李伟[2] 吴刚[1] 蒲云霞[1] 

机构地区：[1]包头医学院,内蒙古包头014060 [2]内蒙古自治区疾病预防控制中心,内蒙古呼和浩特010030

出　　处：《中国卫生检验杂志》2014年第20期2924-2925,2931,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的建立反相高效液相色谱法测定食用油中棉酚含量的方法。方法采用甲醇对食用植物油中的棉酚进行提取,上清液用氮气吹干,用乙腈+0.1%甲酸水(85+15)溶液定容,采用C18柱(250 mm×4.6 mm,5μm)分离,以85%乙腈+0.1%甲酸水溶液为流动相,在235 nm的波长下对游离棉酚进行定性、定量的检测。结果峰面积与游离棉酚的含量在0.5μg/ml^50μg/ml浓度范围内呈良好线性关系,相关系数r=0.99994,回收率在87.1%~100.5%之间,测定相对标准偏差<2%。检出限1.0 mg/kg,定量限3.0 mg/kg。结论该方法操作简便、灵敏、结果准确可靠,适合食用植物油中棉酚的检测。Objective To establish the method for detection of gossypol content in Edible vegetable oil by opposite high per- formance liquid chromatography method （HPLC）. Methods Methanol was used to extract free gossypol in Edible vegetable oil. The supernatant liquid was dried with nitrogen, to make constant -volume with the solution of 85% acetonitrile and 15% formic acid （ 1% concentration）, using Cls column （ particle size 5μm, 4.6 mm × 250 mm） for separation, acetonitrile and for- mic acid buffer solution were used as mobile phase, and made at wavelength 235 nm. Qualitative and quantitative test. Results Peak area and concentration of free gossypol in range 0.5 μg/ml - 50 μg/ml have obvious linear, correlation coeffi- cient（r） is 0. 9994, Recovery of melamine was 87.1% N 100.5% , relative standard deviation （RSD） was less than 2%. The detection limits was 1 mg/kg, quantitative limited to 3.0 mg/kg. Conclusion The proposed method was simple, sensitive and reliable for the determination of free gossvnol.

关 键 词：高效液相色谱法 食用植物油 游离棉酚 

分 类 号：O657.72[理学—分析化学]