脱位牙再植术后龈沟液内MMP-2及TIMP-2含量定量分析  

Quantitative analysis of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 in gingival crevicular fluid of replanted avulsed tooth

作　　者：胡碧波[1] 任雪瑞[1] 魏利敏[1] 王剑锋[1] 

机构地区：[1]温州医科大学口腔医学院.附属口腔医院,浙江温州325027

出　　处：《中国实用口腔科杂志》2014年第9期552-554,共3页Chinese Journal of Practical Stomatology

基　　金：温州市科技计划项目(Y20100167);浙江省大学生科技创新项目(2011R413024)

摘　　要：目的对脱位牙再植术后龈沟液（gingival cervicular fluid,GCF）内基质金属蛋白酶（matrix metalloproteinase,MMP）-2和基质金属蛋白酶组织抑制因子（tissue inhibitor of metalloproteinase,TIMP）-2的含量进行定量分析,并研究术后不同时间段其变化规律,探讨GCF内MMP-2及TIMP-2在牙周组织改建中的作用。方法选择2010年8月至2012年3月在温州医科大学附属口腔医院就诊的20例7~13岁上颌中切牙外伤脱位患儿,参照国际牙外伤协会的年轻恒牙外伤管理指南2（2007年版）中的处理步骤行脱位牙再植术并进行固定,分别于术后第1、3、7天采集再植牙的GCF并定量测定其中MMP-2及TIMP-2的含量,比较不同时间段GCF内MMP-2及TIMP-2含量的差别,并比较MMP-2与TIMP-2比值（MMP-2/TIMP-2）的差别。结果术后第1天GCF内MMP-2及TIMP-2的含量均显著高于第3天和第7天,且术后第1天两者比值亦显著高于第3和第7天。结论脱位牙再植术后前期阶段（1~3 d）GCF中MMP-2及TIMP-2的含量变化剧烈,后期阶段（4~7 d）变化程度较小。提示MMP-2及TIMP-2可能在牙再植后牙周组织改建过程中扮演重要角色。Objective To investigate MMP-2 and TIMP-2 in gingival crevicuiar fluid of replanted avulsed ineisors, and to study the difference at different stages after replantation. Methods Totally twenty replanted avulsed teeth from children aged 7 to 13 were chosen randomly; replant and splint the avulsed teeth according to guidelines for the management of traumatic dental injuries. II. Avulsion of permanent teeth, International Dental Truman Association.MMP-2 and TIMP-2 concentration was measured respectively at the first, third and seventh day after replantation; the difference among three stages were compared. MMP-2：TIMP-2 ratio was also compared among three stages. Results MMP-2 and TIMP-2 concentration at first day was significantly higher than those of third and seventh day; the M MP-2：TIMP-2 ratio at first day was significantly higher than that at third and seventh day. Conclusion MMP-2 and TIMP-2 concentration in replanted avulsed upper incisor dramatically changes in the early stage after replantation, indicting that MMP-2 and TIMP-2 may play an important role in reconstruction of periodontal tissues.

关 键 词：脱位牙 再植术 龈沟液 基质金属蛋白酶-2 基质金属蛋白酶组织抑制因子-2 

分 类 号：R78[医药卫生—口腔医学]