175例急性白血病流式细胞术免疫分型的研究  

Immunophenotyping of 175 Case with Acute Leukemia by Flow Cytometry

作　　者：姚亚洲[1] 郑引索 陈峰云 陈涛[1] 田昱平 李小妮[1] 杨彩霞[1] 张玲[1] 

机构地区：[1]宝鸡市中心医院血液风湿科,陕西宝鸡721008

出　　处：《中国医药指南》2014年第31期33-34,共2页Guide of China Medicine

摘　　要：目的探讨急性白血病流式细胞术免疫分型的意义以及与FAB分型的对照。方法采用流式细胞术三色荧光标记技术和CD_(45)/SC双参数散点图设门,检测175例急性白血病的免疫表型,对抗原表达情况进行分析。结果 FAB分型和免疫分型的相符率90.28%(158/175),175例急性白血病检出B-ALL44例,占25.14%,其主要表达的抗原阳性率从高到低依次为CD_(19)、CD_(22)、CD_(79)a;T-ALL19例,占10.86%,其主要表达的抗原阳性率从高到低依次为HLA-DR、CD_(34)、CD_5、CD_7、cCD_3、CD_8、CD_(38);非M3 AML103例,占58.86%,其主要表达的抗原阳性率从高到低依次为CD_(33)、CD_(117)、CD_(38)、CD_(13)、HLA-DR、MPO、CD_(34)、CD_(123)、CD_(15)、CD_(64);共检出AML-M3患者6例,占0.34%,其主要表达的抗原阳性为MPO、CD_(13)、CD_(33)、CD_(117)、CD_(123)、CD_(64)。结论流式细胞术免疫分型在白血病分型中起重要作用,是FAB分型的补充和修正,提高了急性白血病诊断的准确率,有必要进一步加强流式细胞术免疫分型的规范化和标准化工作。Obiective To investigate the significance of immunophenotype in acute leukemia via flow cytometry, and study the relationship of immunophenotype and FAB phenotype. Methods Immunophenotypes were detected by three-color flow cytometry（FCM ）and CD45/SSC gating in 175 cases of acute leukemia（AL）and antigentic expressions were analysed. Results FAB classification and immunophenotype consistent rate of 90.28%（158/175）, 175 cases of acute leukemia detected in B-ALL44 cases, accounting for 25.14%, antigen positive rate of its main expression in the order of CD19, CD22, CD79a;T-ALL19 cases, accounting for 10.86%, the positive rate of antigen expression and its main descending order of HLA-DR, CD34, CD5, CD7, cCD3, CD8, CD38;non M3 AML103 cases, accounting for 58.86%, its the positive rate of antigen expression in the order of major CD33, CD117, CD38, CD13, HLA-DR, MPO, CD34, CD123, CD15, CD64;were found in AML-M3 6 patients, accounting for 0.34%, and its main antigen expression of MPO, CD13, CD33, CD117, CD123, CD64. Conclusion Immunophenotype Plays an important role in the classification of acute leukemia and is a supplementary and correction of FAB phenotype. It can enhance the exactness of diagnosis and needs to be standardized.

关 键 词：急性白血病 流式细胞术 

分 类 号：R733.71[医药卫生—肿瘤]