缺氧/复氧对体外培养星形胶质细胞脑源性神经营养因子合成及释放的影响  

The expression of brain-derived neurotrophic factor in primary cultured astrocytes subjected to hypoxia /reoxygenation

作　　者：肖君[1] 张函[1] 王伟[1] 谢敏杰[1] 喻志源[1] 何丹[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院神经内科,湖北武汉430030

出　　处：《中风与神经疾病杂志》2014年第10期868-870,共3页Journal of Apoplexy and Nervous Diseases

基　　金：国家自然科学基金(No.81301126和81030021);中国博士后科学基金(No.2013M531699)资助的课题

摘　　要：目的观察缺氧/复氧条件下体外培养星形胶质细胞活力变化及脑源性神经营养因子(BDNF)释放和表达的变化。方法采用原代培养的大鼠皮质星形胶质细胞,实验分为正常组(N)及缺氧/复氧组(H/R)。在缺氧/复氧各个时间点,应用MTT法检测缺氧/复氧时培养星形胶质细胞的活力变化,应用Western blot检测BDNF的表达水平;ELISA检测星形胶质细胞条件培养液上清中BDNF的含量。结果与对照组相比,在缺氧6 h、复氧72 h以内体外培养星形胶质细胞,细胞活力不会发生明显改变,BDNF的释放量无明显变化,但是缺氧/复氧可诱导体外培养星形胶质细胞BDNF表达量的增加。结论单纯的缺氧/复氧条件可影响体外培养星形胶质细胞BDNF的合成,但不足以引起BNDF的释放改变以及细胞的活力变化。Objective To observe the effect of hypoxia /reoxygenation on primary astrocytes viability and the expression of brain-derived neurotrophic factor. Methods Primary cultured rat cortical astrocytes were randomly divided into two groups： normal control group（ N） and hypoxia /reoxygenation group（ H /R）. At different time points,the changes of cell viability were measured by MTT assay. The levels of BDNF protein were analyzed by western blots,and those in astrocyte-conditioned media were measured by enzyme-linked immunosorbent assay（ ELISA）. Results Compared with the control group,6 h hypoxia and reoxygenation within 72 h did not affect the astrocyte viability,and did not induce the release of BDNF,but hypoxia /reoxygenation did enhance BDNF expression in astrocytes. Conclusion Hypoxia /reoxygenation enhances the expression of BDNF in primary cultured astrocyte,but can＇t induce the changes of cell viability and the release of BDNF in vitro.

关 键 词：星形胶质细胞 缺氧/复氧 脑源性神经营养因子 

分 类 号：R734.34[医药卫生—肿瘤]