清肠化湿方联合美沙拉嗪颗粒对溃疡性结肠炎患者血浆及肠黏膜组织IL-17的影响  

Effect of Qingchang Huashi Recipe on IL-17 in the Plasma and Colonic Mucosa of Patients with Ulcerative Colitis

作　　者：陆玥琳[1] 沈洪[1] 姚宏凤[2] 杨旭[3] 

机构地区：[1]江苏省中医院消化科,南京210029 [2]南京市中医院急诊科,南京210001 [3]南京中医药大学研究生院,南京210046

出　　处：《中国中西医结合杂志》2014年第10期1160-1163,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.81072778)

摘　　要：目的检测IL-17在溃疡性结肠炎(ulcerative colitis,UC)患者血浆及结肠组织中的表达水平,探讨清肠化湿方联合美沙拉嗪协同增效的作用机制。方法选择24例轻、中度UC活动期湿热内蕴证患者,对其肠组织标本进行组织学分级,根据随机数字法分为联合组(12例)与西药组(12例),另选择12名体检健康者作为健康组,联合组予清肠化湿方联合美沙拉嗪治疗,西药组予美沙拉嗪口服,疗程3个月。治疗结束后,ELISA法检测血浆IL-17表达水平,免疫组化SP法检测肠黏膜组织中IL-17浸润。结果UC患者血浆及肠组织IL-17表达均高于健康组(P<0.05),且随组织学分级增加而升高。两组患者血浆及肠组织中IL-17表达较治疗前均降低(P<0.05),且联合组患者IL-17表达水平低于西药组(P<0.05)。结论清肠化湿方联合美沙拉嗪治疗可协同增效,通过下调UC患者体内IL-17的表达抑制肠道炎症。Objective To detect the expression level of interleukin 17 （IL-17） in the plasma and colonic mucosa of patients with ulcerative colitis （UC）, and to explore the synergistic mechanism of Qingchang Huashi Recipe （QHR） combined with Mesalazine. Methods Recruited were 24 mild or moderate UC patients of damp-heat inner accumulation syndrome （DHIAS）. Their samples of intestinal tissues were histologically graded. They were assigned to the combination group and the Western medicine （WM） group, 12 in each group. Besides, another 12 healthy volunteers were recruited as the healthy control group. QHR combined Mesalazine were given to patients in the combination group, while those in the WM group took Mesalazine. The therapeutic course for all was 3 months. By the end of treatment the expres- sion level of IL-17 in the plasma and colonic mucosa was detected using ELISA. The infiltration of IL-17 in the intestinal mucosal tissue was detected by immunohistochemical SP method. Results The expres- sion level of IL-17 in the plasma and colonic mucosa was significantly higher in UC patients than in healthy controls （P 〈0.05）. The higher the histological grading the higher the expression level. The expression level of IL-17 in plasma and colonic tissues decreased after treatment in the two treatment groups （P 〈 0.05）. Besides, the expression level of IL-17 was lower in the combination group than in the WM group （P 〈0.05）. Conclusion QHR combined Mesalazine could synergically enhance the effect and effectively inhibit intestinal inflammation through down-regulating the expression of IL-17.

关 键 词：清肠化湿方 溃疡性结肠炎 白介素-17 

分 类 号：R574.62[医药卫生—消化系统]