羟基磷灰石-凝胶支架复合成骨细胞修复兔颅骨缺损的影像学评价  

Imaging evaluation of repairing rabbit skull defect with hydroxyapatite/geltin scaffolds

作　　者：耿海霞[1] Ching-chang Ko 郭秀娟[1] 封伟[1] 吴文[3] 

机构地区：[1]济宁医学院附属医院,山东济宁272000 [2]美国北卡罗莱纳大学UNC牙学院 [3]济宁医学院,山东济宁272000

出　　处：《临床口腔医学杂志》2014年第10期610-612,共3页Journal of Clinical Stomatology

基　　金：山东省自然科学基金(ZR2010HL046);山东省卫生厅科技发展计划项目(2011HZ018)

摘　　要：目的:探索羟基磷灰石/凝胶支架(HAP-GEL)复合成骨细胞在兔颅骨缺损中的修复效果。方法:将HAP-GEL复合物与成骨细胞联合培养,制备兔颅顶骨骨缺损模型,根据植入材料分为3组,分别为HAP-GEL复合成骨细胞、HAP-GEL支架与空白对照。于术后12周通过大体标本观察、X线与CT分析骨缺损修复情况。结果:术后12周影像学评估,空白对照组骨缺损均为明显透射影;HAP-GEL支架复合成骨细胞与HAP-GEL支架组均为低密度影,缺损的边缘略显模糊。结论:HAP-GEL复合成骨细胞修复兔颅骨缺损,可取得较好的修复效果。Objective： To investigate the ability of HAP-GEL / osteoblasts scaffolds to repair cranial bone defect. Method： HAP-GEL and osteoblasts were co-culture.The rabbits cranial parietal bone defect models were prepared.Respec- tively HAP-GEL / osteoblasts scaffolds and HAP-GEL scaffolds were implanted into the cranial bone defects area. After 12 weeks,the result was evaluated by gross inspection and imaging evaluation. Result： Blank defects were significantly ridiolu- cent area in the imaging of 12 weeks, HAP-GEL/osteoblasts and HAP-GEL scaffolds were low density area. Conclusion： The engineering bone constructed by HAP-GEL scaffolds and osteoblasts scaffold can be applied to the repair of bone defect in rabbit.

关 键 词：羟基磷灰石/凝胶 成骨细胞 骨缺损 支架材料 

分 类 号：R783.1[医药卫生—口腔医学] R816.98[医药卫生—临床医学]