雨蛙素及脂多糖在小鼠急性胰腺炎建模中的应用  

Application of caerulein and lipopolysaccharides in creating mouse models of mild or severe acute pancreatitis

作　　者：郑英强[1] 黄娟[2] 曾凡才[3] 周翔宇[1] 

机构地区：[1]四川省泸州医学院附属医院血管甲状腺外科,四川省泸州市646000 [2]四川省泸州医学院病理学教研室,四川省泸州市646000 [3]四川省泸州医学院生物化学教研室,四川省泸州市646000

出　　处：《世界华人消化杂志》2014年第27期4068-4074,共7页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.81100272;四川省科技厅基金资助项目;No.2014JY0004~~

摘　　要：目的:利用雨蛙素(caerulein,CAE)及脂多糖(lipopolysaccharides,LPS)复制典型的轻重型急性胰腺炎(acute pancreatitis,AP)模型.方法:C57小鼠50只,随机分为5组:正常对照组(CON组)、CAE 7次组(CAE 7组)、CAE 7次加LPS组(CAE 7+LPS组)、CAE 13次组(CAE 13组)及CAE 13次加LPS组(CAE 13+LPS组),检测血淀粉酶、脂肪酶水平,组织病理学及透射电镜观察.结果:各实验组酶学水平及病理评分明显高于对照组(淀粉酶含量最低的CAE 7组:27020U/dL±3443 U/dL vs CON组:2696 U/dL±400 U/dL,P<0.01;脂肪酶含量最低的CAE 7组:1379 U/L±283 U/L vs CON组:33 U/L±13 U/L,P<0.01;病理评分最低的CAE 7组:5.8±0.9 vs CON组:0.1±0.3,P<0.01);与CAE 7组相比,CAE 13+LPS组酶学水平及病理评分升高更明显(CAE 13+LPS组淀粉酶:46969U/dL±11852 U/dL vs CAE 7组淀粉酶:27020U/dL±3443 U/dL,P<0.01;CAE 13+LPS组脂肪酶:1962 U/dL±496 U/dL vs CAE 7组脂肪酶:1379 U/dL±283 U/dL,P<0.05;CAE13+LPS组病理评分:11.1±1.1 vs CAE 7组病理评分:5.8±0.9,P<0.05);CAE 13+LPS组较CAE 13组病理评分改变更明显(CAE 13+LPS组:11.1±1.1 vs CAE 13组:10.1±0.99,P<0.05);电镜示CAE 7组细胞线粒体和粗面内质网扩张水肿,CAE 13+LPS组细胞坏死、间质结构破坏.结论:单纯CAE腹腔注射7次可制典型急性水肿型胰腺炎模型,CAE 13次加末次LPS腹腔注射可制典型急性坏死型胰腺炎模型,是研究不同程度AP较理想的模型.AIM： To establish typical mouse models of mild or severe acute pancreatitis induced with caeru- lein （CAE） and/or lipopolysaccharides （LPS）.METHODS： Fifty healthy adult male C57 mice were randomly divided into five groups （with10 mice in each group）： a control group （CON group）, the caerulein 7 group （CAE 7 group）, a caerulein 7 plus LPS group （CAE 7 ＋ LPS group）, a caerulein 13 group （CAE 13 group）, and a caerulein 13 plus LPS group （CAE 13 ＋ LPS group）. All the animals were killed three hours after the last intraperitoneal injection. The pancreas was carefully removed for micro- scopic examination and further observed under a transmission electron microscope （TEM）. Serum amylase and lipase concentrations were assayed.RESULTS： Enzyme levels and pathological score in all the experimental groups were sig- nificantly higher than those in the CON group （amylase lowest CAE 7 group： 27020 U/dL ± 3443 U/dL vs CON group： 2696 U/dL ± 400 U/dL, P 〈 0.01; lipase content lowest CAE 7 group： 1379 U/L ± 283 U/L vs CON group： 33 U/L ± 13 U/L, P 〈 0.01; pathological score low- est CAE 7 group： 5.8 ± 0.9 vs CON group： 0.1 ± 0.3, P 〈 0.01）. Compared with the CAE 7 group, the enzyme levels and pathological score in the CAE 13 ＋ LPS group increased more sig- nificantly （CAE 13 ＋ LPS group amylase： 46969 U/dL ± 11852 U/dL vs CAE 7 group amylase： 27020 U/dL ± 3443 U/dL, P 〈 0.01; CAE13 ＋ LPS group lipase： 1962 U/dL ± 496 U/dL vs CAE 7 group lipase： 1379 U/dL ± 283 U/dL, P 〈 0.05; CAE13 ＋ LPS group pathological score ： 11.1 ± 1.1 vs CAE 7 group pathological score ： 5.8 ± 0.9, P 〈 0.05）. The grade of pathological changes in the CAE 13 ＋ LPS group was significantly high- er than that in the CAE 13 group （CAE 13 ＋ LPS group： 11.1 ± 1.1 vs CAE 13 group： 10.1 ± 0.99, P 〈 0.05）. The ultrastructure of acinar ceils was damaged in the CAE 7 group, and the rough endoplasmic reticulum and mitochondria were markedly swol

关 键 词：雨蛙素 脂多糖 急性水肿型胰腺炎 急性坏死型胰腺炎 

分 类 号：R576[医药卫生—消化系统]