Survivin在后发性白内障动物模型中的表达  

Expression of survivin in animal model of posterior capsule opacification

作　　者：刘淑君[1] 代秀玉[1] 刘少义[1] 王文亭[2] 李元彬[1] 

机构地区：[1]青岛大学医学院附属烟台毓璜顶医院眼科,山东烟台264000 [2]青岛大学医学院附属烟台毓璜顶医院中心实验室,山东烟台264000

出　　处：《山东大学耳鼻喉眼学报》2014年第4期81-84,共4页Journal of Otolaryngology and Ophthalmology of Shandong University

基　　金：烟台市科学技术发展计划项目(2010148-24)

摘　　要：目的建立新西兰大白兔后发性白内障(PCO)动物模型,检测凋亡抑制因子Survivin在PCO中的表达,探讨Survivin在PCO形成过程中的作用。方法选用健康新西兰大白兔30只,随机分为对照组(5只)、实验组(25只)。对照组直接处死;实验组行右眼晶状体超声乳化吸出术,分别于术后即刻,3、7、14、28 d(每个时间点5只)对术眼行裂隙灯显微镜检查,然后处死。获取对照组及实验组右眼撕除部分前囊膜的囊袋,采用Western blotting和逆转录聚合酶链反应(RT-PCR)方法检测Survivin在对照组及实验组不同时间点PCO中的表达。结果 Western blotting和RT-PCR在对照组及实验组术后即刻均未检测到Survivin的表达,实验组术后3、7、14、28 d PCO组织中可检测到不同程度Survivin的表达,其中术后7 d达到高峰,术后14 d表达开始下降。结论 Survivin的表达在PCO的形成过程中具有相应的时相性,提示Survivin与PCO的形成具有一定的相关性。Objective To establish animal model of posterior capsule opacification（ PCO） in New Zealand white rabbits and detect survivin expression in PCO tissue. Methods Thirty adult New Zealand white rabbits were randomly divided into control group and experimental group. The 5 rabbits in control group were executed. Ultrasonic phacoemulsification was performed in the 25 experimental rabbits＇ eyes. Their eyes were examined by slit lamp microscope to observe the development of PCO immediately and 3,7,14 and 28 days after surgery,and then they were executed. The posterior capsule of right eyes was taken in all the rabbits. Western blotting and reverse transcription polymerase（ RT-PCR） were performed to detect survivin expression in PCO at different time points postoperatively. Results Both Western blotting and RT-PCR method indicated that different level of survivin expression was detected in the tissue of PCO 3,7,14 and 28 days after surgery. It reached the peak at the 7th day,and began to decrease at the 14 th day after surgery. Conclusion The survivin expression can be detected in the tissue of PCO in certain time,which indicated that survivin is correlated with the pathogenesis of PCO.

关 键 词：后发性白内障 凋亡抑制因子SURVIVIN 新西兰大白兔 

分 类 号：R776.1[医药卫生—眼科]