WT1基因在白血病与非白血病中的表达差异及其临床意义  

WT1 Gene Expression Difference in Leukemia and Non-Leukemia and Its Clinical Significance

作　　者：刘华胜[1] 朱明山[1] 张海玲[1] 魏双羽[1] 王晓宁[1] 席晓平[1] 余方方[2] 习杰英[1] 王梦昌[1] 张梅[1] 

机构地区：[1]西安交通大学医学院第一附属医院血液内科,陕西西安710061 [2]西安交通大学医学院公共卫生学院地方病研究所,陕西西安710061

出　　处：《中国实验血液学杂志》2014年第5期1217-1221,共5页Journal of Experimental Hematology

基　　金：2011陕西省科学技术研究发展计划项目(2011K13-01-11)

摘　　要：本研究旨在探讨WT1基因在白血病、多发性骨髓瘤和淋巴瘤中的表达与临床意义。应用实时荧光定量PCR(real-time quantitative PCR,RQ-PCR)方法,分析228例西安交通大学医学院第一附属医院血液内科收治的血液肿瘤患者骨髓细胞WT1基因及内参基因(ABL)拷贝数,以NQ比值(WT1基因拷贝数/内参基因拷贝数的公式)计算基因表达水平。结果表明:1男性与女性患者WT1基因表达水平无显著差异(P>0.05);2将患者分为19岁以下患者组、19-50岁患者组和50岁以上患者组,三组间WT1基因表达水平无显著差异(P>0.05);将以上患者重新分类为45岁以上组和45岁以下组,检测结果也无显著差异(P>0.05);3血液肿瘤初诊组与治疗组WT1基因表达水平差异非常显著(P<0.01),但治疗后3年内各阶段WT1基因表达水平无统计学差异(P>0.05),其中白血病初诊组与白血病治疗组之间差异非常显著(P<0.01),非白血病初诊组与治疗组之间无显著差异(P>0.05);4白血病组WT1基因的表达与非白血病组WT1基因表达之间的差异显著(P<0.01),白血病初诊组与非白血病初诊组之间差异非常显著(P<0.01),白血病治疗组与非白血病治疗组之间差异不显著(P>0.05)。结论:WT1基因是白血病患者初诊时预后评估、治疗中微小残留效果检测的可靠指标,尚未发现WT1基因表达水平在非白血病的血液肿瘤如多发性骨髓瘤和淋巴瘤治疗前后存在差异,需进一步观察研究。This study was aimed to investigate the expression level of Wilms＇ tumor 1 （WT1） gene in hematologic neoplasm （leukemia,multiple myeloma and lymphoma） patients and its clinical significance.Real-time quantitative polymerase chain reaction （RQ-PCR） was used to detect the copy number of WT1 gene and reference gene （ALB） in bone marrow cells of 228 patients with hematologic neoplasm in our hospital.The gene expression level was determined by using the ratio of the copy number of WT1 gene and reference gene.The results showed that the WT1 expression level between male and female patients was not statistically significantly different （P 〉 0.05）.All the patients were divided into 3 groups：the group aged under 19,the group aged between 19-50,and the group aged over 50; the WT1 expression level among the three groups were not statistically significantly different （P 〉 0.05）.The above-mentioned patients were redivided into the groups aged under 45 and over 45,the difference between them was not statistically significant（P 〉0.05）.The difference of WT1 expression level between newly diagnosed patients and treated patients with hematologic neoplasm was statistically significant （P 〈 0.01）,but no statistically significant difference of WT1 expression was found（P 〉 0.05） at each stage within 3 years after treatment,however,among them the difference between newly diagnosed leukemia patients and treated leukemia patients was very statistically significant（P 〈 0.01）,while the difference between newly diagnosed and treated non-leukemia patients was not statistically significant（P 〉0.05）.The expression difference of WT1 between leukemia and non-leukemia patients was very statistically significant （P 〈 0.01）,the difference between the newly diagnosed leukemia and non-leukemia patients also was very statistically significant（P 〈 0.01）.The difference of WT1 expression between treated leukemia and non-leukemia patients was not statistically significant （P 〉 0

关 键 词：WT1基因 白血病 多发性骨髓瘤 淋巴瘤 

分 类 号：R733.7[医药卫生—肿瘤] R733.3[医药卫生—临床医学]