急性淋巴细胞白血病患者血清PTK7 mRNA和蛋白表达水平及其临床意义  

PTK7 mRNA and Protein Expression Level in Serum of Patients with Acute Lymphocytic Leukemia and Its Clinical Significance

作　　者：张关亭[1] 张爱琴[2] 

机构地区：[1]河南省中医药研究院附属医院检验科 [2]河南中医学院第一附属医院检验科,河南郑州450000

出　　处：《中国实验血液学杂志》2014年第5期1222-1225,共4页Journal of Experimental Hematology

摘　　要：本研究旨在检测不同亚型急性淋巴细胞性白血病患者血清PTK7 mRNA和蛋白的表达水平,探讨其诊断的临床价值。选择2012年5月-2014年4月在本院诊断为急性淋巴细胞白血病(ALL)患者,按照纳入和排除标准共纳入136例患者,按照细胞形态学将他们分成L1组(n=42)、L2组(n=45)和L3组(n=49),另外选择48例正常儿童为对照组。使用荧光定量PCR检测外周血单个核细胞中PTK7 mRNA转录水平,采用Western blot检测血清中PTK7蛋白表达量。结果表明,L1组PTK7转录水平显著高于正常组(P=0.000)。L2组PTK7转录水平显著高于L1组(P=0.000)。L3组与L2组之间无显著性差异(P=0.123)。L1组患者PTK7蛋白水平显著高于正常组(t=1.564,P=0.000),L2组PTK7蛋白水平显著高于L1组(t=1.437,P=0.003),L3组PTK7蛋白水平与L2组比较无显著差异(t=0.821,P=0.312)。结论:血清PTK7蛋白表达检测具有诊断ALL的潜在临床价值,但对ALL分型不具有型特异性。The purpose of this study was to detect the serum PTK7 level of patients with acute lymphocytic leukemia,and to reveal its clinical value for diagnosis of diseases.A total of 136 patients diagnosed as acute lymphocytic leukemia from May 2012 to April 2014 in our hospital were enroled in this study and were divided into the L1 group （n =42）,L2 （n =45） and L3 group （n =49） according cytomorphology,and 48 normal children were selected as control group.Fluorescence quantitative PCR was used to detect mRNA level of PTK7 in peripheral blood mononuclear ceils,and Western blot was used to detect PTK7 protein expression.The results showed that the PTK7 mRNA level in L1 group was significantly higher than that in normal group （P =0.000）.The PTK7 mRNA level in L2 group was significantly higher than that in the L1 group （P =0.000）.The PTK7 mRNA level in L3 group and L2 group had not significantly different between each other （P =0.123）.Serum PTK7 protein level in L1 group was very significantly higher than that in normal group （P =0.000）.The serum PTK7 protein level in L2 group were very significantly higher than that in the L1 group（P =0.003） and serum PTK7 protein level in L3 and L2 group had no significance difference （P =0.312）.It is concluded that the expression level of serum PTK7 protein has a potential clinical value for the diagnosis of acute lymphocytic leukemia,but without specificity for ALL subsets.

关 键 词：急性淋巴细胞性白血病 酪氨酸蛋白激酶7 MRNA 酪氨酸蛋白激酶7蛋白 

分 类 号：R733.71[医药卫生—肿瘤]