SLC25A38在儿童急性淋巴细胞白血病细胞中的表达  

Expression of SLC25A38 in Leukemic Cells from Children with Acute Lymphoblastic Leukemia

作　　者：陈华英[1] 卢燕燕[1] 温红[2] 鹿全意[1] 张云武[3] 许华曦[3] 

机构地区：[1]厦门大学附属中山医院血液科,福建厦门361004 [2]厦门大学附属第一医院儿科,福建厦门361003 [3]厦门大学神经科学研究所,福建厦门361005

出　　处：《中国实验血液学杂志》2014年第5期1230-1234,共5页Journal of Experimental Hematology

摘　　要：本研究探讨儿童急性淋巴细胞性白血病中SLC25A38表达水平及其临床意义。以23例初治儿童急性淋巴细胞白血病患儿作为实验组,10例非恶性血液病患儿作为对照组。通过Western blot及实时荧光定量RT-PCR方法检测SLC25A38在蛋白和mRNA水平的表达变化。结果表明:23例实验组中SLC25A38蛋白阳性的有8例,阳性率34.78%,10例对照均未检测出SLC25A38蛋白;ALL患儿中SLC25A38蛋白阳性组mRNA的相对表达量为0.4673±0.05344,阴性组为1.296±0.2517;蛋白阳性组较阴性组SLC25A38 mRNA的表达量明显降低(P=0.001);蛋白阴性组与对照组比较表达量无统计学差异(P=0.1097);临床资料分析发现,蛋白阳性组与阴性组在性别比例、免疫分型、初诊时外周血白细胞数、乳酸脱氢酶之间差异具有统计学意义(P<0.05)。结论:SLC25A38蛋白作为一种新发现的蛋白,在初诊儿童急性白血病细胞中过表达,有可能作为急性白血病的一种新的分子标志和潜在的治疗靶点。This study was aimed to investigate the SLC25A38 expression in pediatric patients with acute lymphoblastic leukemia（ALL） and its clinical significance.A total of 23 newly diagnosed ALL pedictric patients were enrolled in test group,10 pediatric patients with non-hematologic malignancies were selected as control group.The expression in protein and mRNA levels of SLC25A38 were detected by Western blot and real-time PCR respectively.The results showed that the SLC25A38 protein was positive in 8 of 23 pediatric ALL patients（34.78%）,while no positive case was found in 10 controls.The relative expression level of SLC25A38 mRNA was 0.4673 ± 0.05344 in SLC25A38-protein positive group of ALL patients,while that was 1.296 ± 0.2517 in SLC25A38-protein negative group of ALL patients.The expression level of SLC25A38 mRNA in SLC25A38-protein positive group was significantly lower than that in negative group（P =0.001）.No statistically significant difference was found in comparison of SLC25A38-protein negative group of ALL patients with the control group（P =0.1097）.The analysis of clinical data showed that there were significantly differences in sex,immunophenotype,initial peripheral white blood cell count and LDH between the SLC25A38-protein positive and SLC25A38-protein negative groups （P 〈0.05）.It is concluded that as a novel protein,SLC25A38 highly expressed in pediatric ALL patients,indicating that SLC25A38 may serve as a molecular marker and potential therapeutic target for acute lymphoblastic leukemia in children.

关 键 词：急性淋巴细胞性白血病 SLC25A38 蛋白表达 

分 类 号：R733.71[医药卫生—肿瘤]