TRAF6,TAK1和TGF-β基因在弥漫大B细胞淋巴瘤患者化疗前后外周血单个核细胞中的表达  

Expression of TRAF-6,TAK1 and TGF-β mRNA in Peripheral Blood Mononuclear Cells of Patients with Diffuse Large B Cell Lymphoma before and after Chemotherapy

作　　者：陈珍珠[1] 尹青松[1] 魏旭东[1] 陈琳[1] 李玉富[1] 

机构地区：[1]郑州大学附属肿瘤医院(河南省肿瘤医院)血液科,河南郑州450008

出　　处：《中国实验血液学杂志》2014年第5期1301-1305,共5页Journal of Experimental Hematology

基　　金：国家自然科学基金(81170520);国家自然科学基金(81000921);河南省卫生厅科研课题(2011010014)

摘　　要：本研究检测弥漫大B细胞性淋巴瘤(DLBCL)患者外周血单个核细胞中TRAF6、TAK1及TGF-β基因在化疗前后的表达变化,探讨化疗对TRAF6/TAK1信号通路活性的影响。采用Ct值比较法,通过SYBR Green II实时定量PCR检测38例DLBCL患者化疗前及化疗2周期后外周血单个核细胞(PBMNC)中TRAF6、TAK1及TGF-β在mRNA的表达水平,并以12例健康人PBMNC作为对照。结果表明:DLBCL患者治疗前后PBMNC中TRAF6、TAK1和TGF-βmRNA表达水平均高于正常人。治疗前患者TRAF6及TAK1基因表达水平与正常人相比未发现统计学差异(P>0.05),治疗后这两基因的表达明显增高,差异具有统计学意义(P<0.05);在此同时治疗后与治疗前相比,这两基因的表达水平也具明显统计学差异(P<0.05),且这两基因的表达水平呈明显正相关,而TGF-β基因治疗后较治疗前明显下降,差异具统计学差异(P<0.05)。结论:DLBCL患者化疗后TRAF6/TAK1信号通路的活性明显增高,而TGF-β基因治疗后则较治疗前明显下降。This study was purposed to detect the expression levels of TRAF6,TAK1 and TGF-β mRNA in peripheral blood mononuclear cell （PBMNC） of patients with diffuse large B cell lymphoma （DLBCL） before and after chemotherapy,and to explore the effect of chemotherapy on the activity of TRAF6/TAK1 signal pathway.The expression levels of TRAF-6,TAK1 and TGF-β mRNA in PBMNC of 38 patients with DLBCL were detected by using the quantitative real time PCR before treatment or after two cycles of chemotherapy,12 healthy people were served as the control.The results showed that the expression levels of TRAF-6,TAK1 and TGF-β mRNA in PBMNC of DLBCL patients＇ were higher than those in healthy people.Before treatment,the expression levels of TRAF-6 and TAK1 mRNA had no significant difference as compared with healthy people （P 〉 0.05） ; after chemotherapy,the expression levels of these two genes significantly increased,and the differences both had statistically significant as compared with healthy people （P 〈 0.05） ; meanwhile the increased expression levels of these two genes after chemotherapy had statistically significant difference as compared with levels before treatment （P 〈 0.05）,and those expression levels were positively correlated.While the expression level of TGF-β mRNA decreased after chemotherapy as compared with level before treatment,and the differences had statistically significantse（P 〈 0.05）.It is concluded that the activity of TRF6/TAK1 signal pathways in PBMNC of DLBCL patients＇ significantly increases after chemotherapy,while the expression level of TGF-β mRNA after chemotherapy is abviously lower than level before treatment.

关 键 词：弥漫大B细胞淋巴瘤 TRAF6 TAK1 TGF-β 信号通路 免疫抑制因子 

分 类 号：R733.1[医药卫生—肿瘤]