新疆家蚕抗菌肽体外抑制人胃癌细胞AGS的增殖  

Inhibition of Cecropin XJ on Proliferation of Human Gastric Cancer AGS Cells

作　　者：吴艳玲[1] 夏丽洁[1] 张富春[1] 

机构地区：[1]新疆生物资源基因工程重点实验室,新疆大学生命科学与技术学院,乌鲁木齐830046

出　　处：《中国细胞生物学学报》2014年第10期1355-1361,共7页Chinese Journal of Cell Biology

基　　金：新疆维吾尔自治区高技术研究发展计划项目(批准号:201110101)资助的课题~~

摘　　要：新疆家蚕抗菌肽（Cecropin XJ）具有抑制肿瘤细胞生长的能力。为研究不同浓度Cecropin XJ体外对人胃癌细胞AGS生长的影响,分别采用MTT比色法、软琼脂集落形成实验、流式细胞术、划痕愈合和Transwell实验进行检测。结果表明,20-100μg/mL的Cecropin XJ能够显著抑制AGS细胞的增殖,并具有剂量和时间依赖性。同时,20,50,100μg/mL的Cecropin XJ处理后集落形成率分别降低了（35.81±13.10）%、（48.12±5.68）%和（81.46±6.21）%。AGS细胞经不同浓度Cecropin XJ处理24 h后,凋亡率逐渐上升并且细胞周期阻滞于S期。20μg/mL Cecropin XJ能够显著抑制AGS细胞的迁移和侵袭,100μg/mL Cecropin XJ几乎完全抑制AGS细胞的迁移和侵袭。以上结果说明,Cecropin XJ能够抑制AGS细胞生长、增殖,有可能成为人胃癌治疗的辅助药物。Cecropin XJ, an antimicrobial peptide from Xinjiang silkworm, can inhibit tumor cells growth. To investigate the influence of different concentrations of Cecropin XJ on the growth of human gastric cancer AGS cells, several assays including MTT assay, soft agar colony formation assay, flow cytometry, wound-healing and Transwell assay were conducted. The results showed that 20-100 μg/mL of Cecropin XJ could significantly inhibit the AGS cells proliferation in a dose-dependent and time-dependent manner. After treated with 20, 50, 100 μg/mL of Cecropin XJ, colony forming ability was decreased by（35.81±13.10）%,（48.12±5.68）% and（81.46±6.21）%, respectively. The apoptosis rate of AGS cells increased gradually and the cell cycle was arrested in S phase after treated with different concentrations of Cecropin XJ for 24 h. 20 μg/mL of Cecropin XJ could decrease AGS cells migration and invasion, while 100 μg/mL of Cecropin XJ almost completely inhibited AGS cells migration and invasion. In conclusion, Cecropin XJ can significantly inhibit the growth and proliferation of AGS cells and might be potential for the treatment of human gastric cancer.

关 键 词：新疆家蚕抗菌肽 人胃癌细胞AGS 增殖抑制 细胞凋亡 细胞周期 侵袭和转移 

分 类 号：R735.2[医药卫生—肿瘤]