SFRP1基因启动子甲基化在胰腺癌患者血清中的检测及临床意义  

Detection and significance of serum SFRP1 promoter methylation in patients with pancreatic cancer

作　　者：刘畅[1] 袁柏思[1] 陆恒[1] 吴琳[1] 路又可[1] 汪芳裕[1] 

机构地区：[1]南方医科大学附属南京军区南京总医院消化内科,江苏南京210002

出　　处：《胃肠病学和肝病学杂志》2014年第10期1213-1216,共4页Chinese Journal of Gastroenterology and Hepatology

基　　金：国家自然科学基金青年基金项目(81302162);南京军区南京总医院院管基金(2011052)

摘　　要：目的研究分泌型卷曲相关蛋白1(secreted frizzled-related protein 1,SFRP1)基因启动子区异常甲基化与胰腺癌发生的相关性。方法选择胰腺癌患者22例为实验组,另选择健康体检者24名为对照组,提取外周血清DNA,利用甲基化特异性PCR法(MSP)检测SFRP1基因启动子区域甲基化状态。结果在22例胰腺癌患者中,7例存在SFRP1基因甲基化现象,甲基化阳性率为31.8%;而在24名健康体检者血清中仅1人检出SFRP1甲基化,甲基化阳性率为4.2%,两者差异有统计学意义(P<0.05)。SFRP1基因甲基化与患者年龄、性别及血清CA19-9水平无相关性。联合检测血清SFRP1甲基化及CA19-9水平可显著提高胰腺癌检出率。结论血清SFRP1基因启动子区异常甲基化可能参与胰腺癌的发生,并为疾病的早期监测提供可能的分子理论依据。Objective To study the correlation between secreted frizzled-related protein 1 （SFRP1）gene promoter hypermethylation and pancreatic cancer.Methods Twenty-two patients with pancreatic cancer served as the experimental group and 24 healthy subjects served as a control group in this study.DNA was extracted from peripheral blood.Promoter methylation status of SFRP1 gene was determined by methylation-specific polymerase chain reaction （MSP）.Resuits The positive methylation rate of SFRP1 gene was 31.8％ （7/22） in pancreatic cancer patients,which was higher than that in the controls [4.2％ （1/24）,P ＜0.05].SFRP1 gene hypermethylation status was not correlated with age,gender and serum CA19-9 levels of pancreatic cancer patients.Combined analysis of SFRP1 methylation and serum CA19-9 level could increase the detection rate for diagnosis of pancreatic cancer.Conclusion The SFRP1 gene promoter hypermethylation may be involved in the occurrence of pancreatic cancer,thus providing the molecular evidence in theory for its early monitoring.

关 键 词：胰腺癌 分泌型卷曲蛋白1(SFRP1) 甲基化 

分 类 号：R735.9[医药卫生—肿瘤]