芡实对糖尿病肾病大鼠肾组织中SOCS-3及IGF-1表达的影响  

Effect of Euryale Ferox on the Expression of SOCS-3、IGF-1 in Renal Tissues of Diabetic Nephropathy Rats

作　　者：韩利梅[1] 方敬爱[1] 孙艳艳[1] 张晓东[1] 刘文媛[1] 

机构地区：[1]山西医科大学第一医院肾内科,太原030001

出　　处：《中国中西医结合肾病杂志》2014年第9期767-769,I0003,I0004,共5页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：国际科技合作项目(No.2011081066)

摘　　要：目的:探讨芡实对糖尿病肾病(diebetic nephrothy,DN)大鼠肾组织中细胞因子信号抑制因子-3(suppressor of cytokine signaling-3,SOCS-3)及胰岛素样生长因子-1(insulin like growth factor-1,IGF-1)表达的影响及可能机制。方法:采用单剂量腹腔注射链脲佐菌素(STZ,45 mg/kg)建立大鼠DN模型,将DN模型大鼠随机分为5组:DN组(DN组)、小剂量芡实组(EL,1.5 g·kg-1·d-1)、中剂量芡实组(EM,3.0 g·kg·-1d-1)、大剂量芡实组(EH,6.0 g·kg-1·d-1)及氯沙坦钾组(LP,30 mg·kg-1·d-1);另设正常对照组(NC组),每组10只。分别治疗12周后测定各组大鼠生化指标;HE、Masson、PAS染色及电镜观察肾组织病理变化;免疫组化、Western blot法检测肾组织中SOCS-3与IGF-1表达情况。结果:12周末,与NC组相比,DN组大鼠24 h尿蛋白定量(24 h Upr)、尿素氮(BUN)及血肌酐(Scr)均明显升高(P<0.05),肾小球体积增大、系膜细胞增生、基质增多,肾小管扩张,肾组织中SOCS-3表达增加、IGF-1表达明显增加(P<0.05);与DN组相比,LP组与EM组大鼠24 h Upr、BUN及Scr明显降低(P<0.05),病理改变减轻,肾组织SOCS-3表达明显增加、IGF-1表达明显下降(P<0.05);LP组与EM组间降尿蛋白作用差异无统计学意义。结论:芡实可能通过上调SOCS-3表达,抑制IGF-1过表达进而减少蛋白尿,发挥肾脏保护作用。Objective：To investigate the effect and mechanism of euryale ferox on the expression of SOCS-3 and IGF-1 in renal tissues of diabetic nephropathy（ DN） rats. Methods：The rat models of DN established by a single injection of streptozotocin were randomly divided into 5 groups, namely diabetic nephropathic model group （ group DN） , low-dose Euryale ferox group （ EL, 1.5 g·kg-1·d-1）, medium -dose Euryale ferox group （EM, 3. 0 g·kg-1·d-1）, high -dose Euryale ferox group （EH, 6. 0 g·kg-1·d-1） and losartan potassium group （LP, 30 mg·kg-1·d-1）. With normal rats as the contro1, each group of 10. All the rats received daily gavage for 12 weeks, 24 h urinary protein （24 hUpr）, blood urea nitrogen （BUN） and blood serum creati-nine （ Scr） were measured after the research. The renal pathological changes were observed by histochemistry staining and electron microscope. The expression of SOCS-3 and IGF-1 was detected by immunohistochemistry and western blot. Results：Compared with group NC, there were the glomerular hypertrophy, proliferation of mesangial cells, the accumulation of mesangial matrix and renal tu-bular expansion in group DN. 24 h Upr, BUN and Scr also increased significantly （P〈0. 05）. Meanwhile the expression of SOCS-3 and IGF-1 in renal tissues increased significantly （P〈0. 05）. Compared with group DN, the pathology changes were relieved, 24 h Upr, BUN, Scr, as well as the expression of IGF-1 decreased and SOCS-3 increased discinctly in group EM and group LP （P〈0. 05）. In addition, there was no significant difference in effect of reducing urinary protein in group EM and LP. Conclusion：Euryale ferox may reduce proteinuria and protect renal function by up-regulating the expression of SOCS-3, inhibiting the overex-pression of IGF-1.

关 键 词：糖尿病肾病 芡实 SOCS-3 IGF-1 

分 类 号：R285.5[医药卫生—中药学]