液泡膜-ATP酶与P-糖蛋白在结肠癌组织中的表达及其临床意义  

Expression of V-ATPase and P-gp in colon carcinoma tissues and its significance

作　　者：赵加应[1] 蔡元坤[1] 李小静[2] 殷琛庆[1] 舒维清[2] 武景波[2] 朱卫平[1] 

机构地区：[1]复旦大学附属上海市第五人民医院普外科,200240 [2]复旦大学附属上海市第五人民医院病理科,200240

出　　处：《中华普通外科杂志》2014年第10期771-774,共4页Chinese Journal of General Surgery

基　　金：上海市卫生和计划生育委员会基金资助项目（20134260）;上海市闵行区科委基金资助项目（2012MHZ021）

摘　　要：目的 探讨液泡膜-ATP酶（Vacuolar-H＋-ATPase,V-ATPase）与P-糖蛋白（P-glyeoprotein,P-gp）在结肠癌组织中的表达及其临床意义.方法 应用免疫组织化学方法检测80例结肠癌组织、20例结肠腺瘤组织和10例正常结肠黏膜组织中V-ATPase与P-gp蛋白的表达,分析它们的相关性及其与结肠癌患者的临床病理特征和预后的关系.结果 V-ATPase和P-gp蛋白在结肠癌组织中阳性表达率（72％和80％）高于结肠腺瘤组阳性表达率（40％和35％）及正常结肠黏膜组阳性表达率（20％和20％）,差异均有统计学意义（均P＜0.05）.结肠癌组织中V-ATPase蛋白表达与P-gp蛋白表达呈正相关（r=0.567,P＜0.01）,V-ATPase蛋白和P-gp蛋白的表达与肿瘤的TNM分期、淋巴结转移和肿瘤分化程度有关（均P＜0.05）.V-ATPase蛋白高表达结肠癌患者的5年生存率低于低表达者（P=0.023）,5年复发率高于低表达者（P=0.024）.结论 V-ATPase在结肠癌组织中表达上调,与结肠癌的侵袭和转移有关,V-ATPase蛋白高表达患者的预后不良.Objective To investigate the expression of Vacuolar-H ＋-ATPase （V-ATPase） and P-glyeoprotein （P-gp） protein in colon carcinoma tissues,the correlation between the expression of V-ATPase and P-gp and their clinicopathological significance.Methods In samples from 80 cases of colon cancer,20 cases of colon adenoma and 10 cases of normal colonic mucosa tissues,the expression of V-ATPase and P-gp protein were detected by immunohistochemical method,their relationship was analyzed,the clinicopathological features and prognosis were evaluated.Results In colon cancer,V-ATPase and P-gp protein expression was 72％ and 80％,higher than that in colon adenomas （40％,35％）,and in normal colon mucosa （20％,20％）,the difference was statistically significant （P ＜ 0.05）.There was a positive correlation between the expression of V-ATPase and that of P-gp （r =0.567,P ＜0.01）.V-ATPase and P-gp protein expression in colon cancer was associated with TNM stage,lymph node metastasis and tumor differentiation （P ＜ 0.05）.Patients with high V-ATPase expression had lower 5-year survival rate than those with low V-ATPase expression （P =0.023）,and 5-year recurrence rate was higher than those with low expression （P =0.024）.Conclusions The expression of V-ATPase is up-regulated in colon cancer,there is a positive correlation with colon cancer progress and metastasis,and high V-ATPase protein expression predicts poor prognosis.

关 键 词：结肠肿瘤 P糖蛋白类 腺苷三磷酸酶类 肿瘤微环境 

分 类 号：R735.35[医药卫生—肿瘤]