纯钛表面加载RGD多肽的体外实验研究  

Study of pure titanium surface loaded with RGD peptide in vitro

作　　者：徐基亮[1] 夏荣[1] 孙磊[1] 胡小晔[2] 刘春[1] 孙子环 

机构地区：[1]安徽医科大学第二附属医院口腔科,合肥230601 [2]安徽省纳米材料与纳米结构重点实验室,中国科学院合肥物质研究院固体物理研究所,合肥230031

出　　处：《安徽医科大学学报》2014年第11期1591-1595,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽省科技厅年度重点科研资助项目(编号:12070403070);安徽省学术和技术带头人科研活动经费资助(编号:2014H030)

摘　　要：目的探讨纯钛表面加载RGD多肽进行生物功能化修饰的可行性。方法借助电化学和分子自组装技术在纯钛表面构建TiO2纳米管-聚多巴胺-RGD多肽活性层,采用场发射扫描电镜(FESEM)、X射线光电子能谱(XPS)对各组钛片进行表面形貌和元素组成分析。培养小鼠骨髓基质干细胞(BMSCs),对处理前后的钛片进行体外生物活性评价。结果 SEM和XPS显示,两步阳极氧化后在纯钛表面形成蜂窝多孔的TiO2纳米管,多巴胺在TiO2纳米管上自聚合成聚多巴胺涂层,同时向外接枝偶联RGD多肽,最终在纯钛表面成功形成了TiO2纳米管-聚多巴胺-RGD多肽活性层,体外细胞培养显示,该活性层有利于细胞黏附、增殖和分化。结论 RGD多肽功能化修饰的钛片有良好的生物相容性,这种钛表面功能化修饰法可望用于改善钛种植体,提高骨结合。Objective To investigate the feasibility of pure titanium surface functionalization with RGD peptide. Methods TiO2 nanotubes-polydopamine-RGD peptide bioactive layers were built on titanium surface with electro- chemistry and molecular self-assembly technique. Titanium surface morphology and elemental composition analysis of each group were characterized by field emission scanning electron microscopy （FESEM） and X-ray photoelectron spectroscopy （XPS）. Mouse bone marrow stromal cells （BMSCs） were cultured in vitro to evaluate biological activ- ity of titanium before and after treatment. Results SEM and XPS showed that honeycomb porous TiO2 nanotubes were formed on pure titanium after the two-step anodization, dopamine self-polymerized to a polydopamine film onto surfaces of TiO2 nanotubes, polydopamine film was then utilized to conjugate RGD, finally forming TiO2 nanotubes- polydopamine-RGD peptide bioactive layers . In vitro cell culture showed that the active layer was conducive to cell adhesion, proliferation and differentiation. Conclusion Titanium disks of functional modification with RGD pep- tide have good biocompatibility, which could be used for developing the titanium implants and further to improve os-seointegration.

关 键 词：钛 TIO2纳米管 RGD多肽 多巴胺 骨髓基质干细胞 

分 类 号：R783.1[医药卫生—口腔医学]