急性低压低氧对小鼠学习记忆及海马ACh水平、磷酸化tau蛋白表达的影响  

Effects of acute hypobaric hypoxia on learning memory behaviors and the level of acetylcholine,expression of phosphorylated tau protein in hippocampus of mice

作　　者：钟玲[1] 宋永斌[1] 徐建春[1] 焦岩[1] 王荣[1] 杨遥[1] 刘静[1] 徐江涛[1] 

机构地区：[1]兰州军区乌鲁木齐总医院神经内科,乌鲁木齐830000

出　　处：《安徽医科大学学报》2014年第11期1610-1613,共4页Acta Universitatis Medicinalis Anhui

基　　金：全军"十一五"计划课题项目(编号:06MB106);全军心理卫生应用性科研课题项目(编号:12XLZ302)

摘　　要：目的探讨模拟高原急性低压低氧环境对小鼠学习记忆行为及乙酰胆碱水平(ACh)、磷酸化tau蛋白(P-tau)表达的影响。方法将小鼠置于模拟海拔6 000 m的低压低氧舱内8 h后,用Morris水迷宫测定小鼠的空间学习记忆能力;用比色法测定海马ACh水平和胆碱乙酰转移酶(ChAT)活性的变化;用免疫组织化学方法观察其海马区总tau蛋白(T-tau)和P-tau表达的变化。结果与常压常氧组比较,低压低氧组小鼠寻找平台的潜伏期延长(P<0.01),目标象限停留时间减少(P<0.05),海马区ACh水平和ChAT水平显著下降(P<0.05,P<0.01)P-tau表达增加(P<0.05)。结论急性低压低氧环境可使小鼠学习记忆能力下降,这可能与海马区ACh水平和ChAT减少以及P-tau表达增加有关。Objective To examine the effects of acute hypobaric hypoxia on the capabilities of learning and memo- ry, the level of acetylcholine and the expression of P-tau in hippocampus of mice. Methods The acute hypobaric hypoxia environment were imitated by putting the mice in a hypobaric chamber at altitude of 6 000 meters for 8 hours. Morris water maze test to detect the capabilities of learning and memory of mice. The level of ACh and the ac- tivity of ChAT in hippocampus were examined by colorimetric method and the expression P-tau and T-tau were ex- amined by immunohistochemistry. Results The mean escape latency of mice in the hypobaric hypoxia group were longer than the normobaric normoxia group（ P 〈 0. 01 ）, and the target quadrant residence time were decreased（ P 〈 0. 05 ）. Compared with the normobaric normoxia group, level of ACh and the activity of ChAT in hippocampus of mice in the hypobaric hypoxia group were all reduced （P 〈 0. 05 ＆ P 〈 0.01 ） , otherwise, the expression of P-tau in hippocampus of mice were increased. Conclusion Acute hypobaric hypoxia environment can decrease the abilit ies of learning and memory of mice, and these changes might be related with the reduction of the level of ACh and the expression of P-tau in hippocampus of mice.

关 键 词：低压低氧 学习记忆 海马 乙酰胆碱 磷酸化TAU蛋白 

分 类 号：R741[医药卫生—神经病学与精神病学]