骨髓间充质干细胞联合维生素E对急性肾损伤炎症反应的抑制作用  

Effect of bone marrow-derived mesenchymal stem cells combined with vitamin E on inflammatory reaction in acute kidney injury

作　　者：赵雷 冯业童[2] 董超[2] 周余来[2] 刘朋飞[2] 

机构地区：[1]暨南大学第二临床医学院深圳市人民医院麻醉科,广东深圳518020 [2]吉林大学药学院再生医学系,吉林长春130021

出　　处：《中国病理生理杂志》2014年第10期1891-1895,1901,共6页Chinese Journal of Pathophysiology

基　　金：深圳市知识创新计划(No.201404163000377);吉林大学研究生创新基金资助项目

摘　　要：目的:探索骨髓间充质干细胞联合维生素E对急性肾损伤中炎症反应的抑制效果,开发针对急性肾损伤的新型治疗模式。方法:采用庆大霉素作为急性肾损伤药物,复制大鼠急性肾损伤模型,以骨髓间充质干细胞联合维生素E对急性肾损伤进行治疗,治疗结束后,收集大鼠的血浆和肾脏组织,采用实时荧光定量PCR及ELISA方法,评价血浆及组织中炎症蛋白的表达。结果:通过骨髓间充质干细胞联合维生素E对大鼠急性肾损伤模型进行治疗后,大鼠血浆中的炎症蛋白显著降低;在肾脏组织中,与单一应用骨髓间充质干细胞或维生素E相比,二者联合在某些炎症蛋白的抑制方面具有更明显的效果。结论:骨髓间充质干细胞联合维生素E对急性肾损伤中炎症反应具有一定抑制效果,优于单一骨髓间充质干细胞或单一维生素E的治疗方式,所以,这种联合治疗模式在急性肾损伤的治疗方面更具有临床应用价值。AIM： To explore the effect of bone marrow-derived mesenchymal stem cells（ BMSCs） combined with vitamin E on the inflammatory reaction in acute kidney injury（ AKI） rats. METHODS： Gentamicin was used to induce AKI and the rats were treated with BMSCs combined with vitamin E. After treatment,the rat plasma and kidney tissues were collected,and the expression of inflammatory factors at mRNA and protein levels was detected by real-time quantitative PCR and ELISA. RESULTS： After the treatment with BMSCs combined with vitamin E,the inflammatory proteins were down-regulated in the plasma and the renal tissues. Compared with single treatment group,the decreases in the inflammatory proteins were more obvious in combined treatment group. CONCLUSION： The method of BMSCs combined with vitamin E takes the anti-inflammatory effect on AKI,indicating a new and potential mode in clinical application for AKI therapy.

关 键 词：骨髓间充质干细胞 维生素E 急性肾损伤 炎症反应 

分 类 号：R363[医药卫生—病理学]