肝脏缺血再灌注对肝癌肝内转移和生长作用的研究  

Study of the role of liver ischemia/reperfusion in intrahepatic metastasis and growth of liver tumor

作　　者：王进 周丁华 刘毅[2] 董家鸿[3] 

机构地区：[1]第二炮兵总医院肝胆外科,北京100088 [2]山东大学齐鲁医院肝胆外科,山东济南250012 [3]中国人民解放军总医院,北京100853

出　　处：《中国现代普通外科进展》2014年第9期688-691,共4页Chinese Journal of Current Advances in General Surgery

基　　金：国家传染病科技重大专项资助(2008ZX10002-26)

摘　　要：目的:探讨缺血再灌注时间对肝癌肝内转移生长的作用。方法:建立70%缺血再灌注模型肝癌肝内转移小鼠模型。分为四组:假手术组,缺血15min组,缺血30 min组,缺血45 min组。四组都要在再灌注后注射肿瘤细胞,观察小鼠的生存时间,肿瘤大体观察,肿瘤组织切片HE染色计算肿瘤肝组织被替代区域(HRA)。结果:缺血30 min组和缺血45 min组肿瘤肝脏肝组织被替代区域更大,小鼠生存时间更短。结论:缺血30 min、缺血45 min组肝损伤相对更重,肝内肿瘤转移生长更快。Objective： It is reported that hepatic ischemia/reperfusion（I/R） during hepatectomy accelerates liver tumor growth,The aim of this study was to examine the whether the time of is- chemia/reperfusion can influence the liver tumor metastases and growth. Methods： A murine tu-mor model of ischemia/reperfusion was divided into four groups： sham operation, 15-min period of 70% partial live ischemia, 30-min period of 70% partial live ischemia, 45-min period of 70% partial live ischemia. Results： In those mice subjected to 30 and 45 min of 70% hepatic I/R, there is a sig- nificant HRA increase, lower survival time and higher ALT levels when compared with mice receiving tumor injection and laparotomy alone. CoNCLUSION： There were significant liver injury and increased tumor metastasis and growth after ischemia of more than 30 rain. The mice with tumor cell injection had a lower survival time after ischemia of more than 30 min.

关 键 词：缺血再灌注 肝肿瘤 转移 

分 类 号：R735.7[医药卫生—肿瘤]