不同采血方法对大鼠内分泌及血常规相关指标的影响  

Effects of Different Blood Sampling Methods on Endocrine and Haematological Parameters in Rats

作　　者：吴剑平[1] 鞠晓云[1] 范乃兵[1] 

机构地区：[1]南京中医药大学实验动物中心,南京210032

出　　处：《实验动物与比较医学》2014年第5期377-380,共4页Laboratory Animal and Comparative Medicine

基　　金：南京中医药大学校自然科学基金（项目编号：012072004030）

摘　　要：目的 比较剪尾、尾静脉切割和眼眶穿刺3种采血对大鼠内分泌及血常规指标的影响,探讨适用于大鼠连续多次采血的优选方法.方法 选择剪尾、尾静脉切割和眼眶穿刺3种采血方法,分别在0 min、20 min、60 min、120 min时间点对Wistar大鼠进行4次采血,测定各组皮质酮（CORT）、血糖（GLU）及血常规指标,并与常规饲养不予任何实验处理作为空白对照组.分析不同采血方法对大鼠上述指标的影响.结果 与空白对照组相比,3个采血组的血清CORT均有显著升高,同时眼眶穿刺采血组CORT显著高于其他两组.4次采血过程中,剪尾采血和尾静脉切割采血对GLU均有一定影响,但眼眶穿刺采血会造成GLU有超过3 mmol/L的大幅波动.三个采血组比较,眼眶穿刺采血组的初始GLU显著高于其他两组,同时三组之间白细胞总数（WBC）、红细胞数（RBC）、血红蛋白含量（HGB）、红细胞压积（HCT）和血小板数（PLT）等血常规指标均有显著性差异.结论 尾静脉切割和剪尾采血为相对优化的采血方法,适用于大鼠连续多次采血,口服葡萄糖耐量试验（OGTT）不宜采用眼眶穿刺采血.不同采血途径会造成血常规和血糖测定值的明显差异.Objective To select refined methods from the three methods of amputation of the tail tip, lateral tail incision and periorbital puncture, for multiple blood sampling in rats. Methods Three methods of amputation of the tail tip, lateral tail incision and periorbital puncture, were selected for blood sampling on Wistar rats. For each methods, four samples were drawn at 0rain, 20rain, 60rain and 120min. Indexes of CORT, blood glucose and haematological parameters were measured to study the effects on rats. Results Compared with control group, CORT significant increased in the three blood sampling groups. CORT in the group of periorbital puncture was significant higher than in the other blood sampling groups. During the four samplings, amputation of the tail tip and lateral tail incision all influ- enced blood glucose,while periorbital puncture resulted in a dramatic increase in blood glucose of up to 3 mmol/L. Compared the three blood sampling groups, the initial blood glucose in the group of periorbital puncture was significant higher than the other two groups, while there were significant differences in the haematological parameters of WBC, RBC, HGB, HCT and PLT. Conclusion For the three methods, amputation of the tail tip and lateral tail incision caused smaller stress reaction than periorbital puncture, which relatively be refined methods for multiple blood sampling. Furthermore, periorbital puncture should not be applied for OGTT in rats.Blood samples drawn by different blood sampling methods may induce significant differences in the haematological parameters and blood glucose.

关 键 词：大鼠 多次采血 内分泌指标 血常规 

分 类 号：R-332[医药卫生]