Poly I∶C刺激对裸鼹鼠体内自噬水平的影响  

The Effect of Poly I∶C on Autophagy of Naked Mole Rat

作　　者：林丽芳[1] 肖邦[1] 赵善民[1] 汤球[1] 余琛琳[1] 孙伟[1] 张璐[1] 蔡丽萍[1] 徐晨[1] 崔淑芳[1] 

机构地区：[1]第二军医大学实验动物中心,上海200433

出　　处：《实验动物与比较医学》2014年第5期411-416,共6页Laboratory Animal and Comparative Medicine

基　　金：上海市科技发展基金项目（No.12140900200）、（No.13140900400）和（NO.31402028）联合资助

摘　　要：目的 研究多聚肌苷-多聚胞苷酸钠盐（Poly I∶C）对裸鼹鼠自噬水平的影响.方法 成年裸鼹鼠以及C57BL/6小鼠分别随机分成Poly I∶C给药组和对照组,分别腹腔注射Poly I∶C和生理盐水.Poly I∶C给药12h后,组织切片HE染色观察小肠组织的病理变化,电镜观察细胞结构的变化,实时定量PCR检测小肠组织中炎症相关因子IL-1、ATG5的mRNA表达情况,蛋白印迹检测小肠组织中自噬相关蛋白LC3B、Beclin 1表达情况.结果 Poly I∶C给药后,裸鼹鼠小肠组织未出现显著的病理变化,但C57BL/6小鼠小肠组织腺体部出现广泛性出血;电镜观察发现,裸鼹鼠小肠组织细胞中线粒体增多、变长,自噬体数量显著增加,而小鼠组织细胞线粒体有大量的空泡化,滑面内质网扩张;实时定量PCR检测未发现组织中IL-1、ATG5 mRNA表达有显著变化;蛋白印迹检测发现,给药后裸鼹鼠组织中LC3B蛋白表达显著上调（P＜0.05）,而C57BL/6小鼠则下调（P＜0.05）.结论 Poly I∶C给药引起裸鼹鼠自噬水平上调,同时,裸鼹鼠自噬水平的上调亦可以提高机体的适应能力,进而起到抵抗Poly I∶C损伤的作用.Objective To study the effect of polyinosinic-polycytidylic acid（Poly I： C） on the autophagy in naked mole rat. Methods The adult naked mole rats and C57BL/6 mice were randomly divided into Poly I： C treatment group （ip Poly I： C） and control group （ip saline）. Twelve hours after Poly I： C administered, the differences in the intestinal pathological changes and subcellular organelles between naked mole rats and C57BL / 6 mice were observed by light and electron microscopy respectively. The mRNA expression of inflammation-related factor IL-1 and autophagy-related gene ATG5 in intestinal tissue were detected by quantitative real-time PCR. Detection of autophagy-related proteins LC3B and Beclin 1 was conducted by Western blot. Results Naked mole rats did not show significant pathological changes after Poly I： C treatment, but intestinal tissue glands of C57BL/6 mouse show serious hemorrhage. Further observation by electron microscope found that the mitochondria＇s size was elongagted, number increased and more autophagy in naked mole rats＇ intestinal tissue cells. Meanwhile, a lot of vacuolatation of mitochondria, dilation of smooth endoplasmic reticulum was observed in mouse intestinal tissues. Significant difference was not found in mRNA expression of IL-1, ATG5 by real-time quantitative PCR. In Western blot detection, LC3B protein expression was significantly increased （P〈0.05） in naked mole rats, after administration, but that of C57BL/6 mice were reduced （P〈0.05）. Conclusion Poly I： C administration induced upregulation of autophagy in naked mole rat. While, autophagy may regulate the inflammatory response in turn, which played an important role in naked mole rats＇ anti-Poly I：C injury.

关 键 词：裸鼹鼠 多聚肌苷-多聚胞苷酸钠盐(Poly I∶C) 炎症 自噬 

分 类 号：R-332[医药卫生]