抑癌基因p53在裸鼹鼠不同组织中表达水平的差异  

Expression of Tumor Suppressor Gene p53 in Different Tissues of Naked Mole Rat

作　　者：赵善民[1] 肖邦[1] 王运慧[1] 林丽芳[1] 余琛琳[1] 张璐[1] 宫晨[1] 汤球[1] 孙伟[1] 崔淑芳[1] 

机构地区：[1]第二军医大学实验动物中心,上海200433

出　　处：《实验动物与比较医学》2014年第5期428-432,共5页Laboratory Animal and Comparative Medicine

基　　金：国家自然科学基金项目（No.31402028）与上海市科技发展基金项目（No.13140900400）联合资助

摘　　要：目的 比较裸鼹鼠与C57BL/6J小鼠组织中抑癌基因p53表达的差异,并分析p53基因在不同年龄裸鼹鼠不同组织中表达水平的差异.方法 采用Western blotting检测新生裸鼹鼠与C57BL/6J小鼠肝脏、肺脏、脑、肾脏中p53蛋白的表达,进一步比较了不同年龄裸鼹鼠肝脏、肺脏、脑、肠道中p53蛋白的表达,同时采用RT-PCR检测了裸鼹鼠肝脏、肺脏中p53基因的转录水平.以及低氧处理裸鼹鼠皮肤成纤维细胞中p53基因的表达水平.结果 新生裸鼹鼠肝脏、肺脏、脑、肾脏组织中p53蛋白的表达低于新生C57BL/6J小鼠组织,成年裸鼹鼠肝脏、肺脏组织中p53蛋白表达量亦显著高于幼年裸鼹鼠.裸鼹鼠成纤维细胞在低氧条件下p53蛋白的表达量较高,并且随着低氧处理时间的延长,表达量进一步升高.结论 新生裸鼹鼠组织中抑癌基因p53表达水平显著低于C57BL/6J小鼠,并且p53基因随着年龄增长而发生变化.Objective To compare the expression of tumor suppressor gene p53 in different tissues of 1-day-old naked mole rat and 1-day-old C57BL/6J mice and analyze p53 expression in different tissues of naked mole rats with different age. Methods The p53 protein expression from liver, lung, brain and kidney of newborn naked mole rats and C57BL/6J mice were determined by Western blotting. Then the p53 protein expression from liver, lung, brain and intestinal tissues from naked mole rats of different age were determined. The p53 mRNA of livers and lungs from naked mole rats were determined by RT-PCR. Exposure of cells to hypoxia increased the p53 protein expression in a time-dependent manner. Results Expression of p53 protein in the livers, lungs, brain, and intestinal tissues of the naked mole rat were lower than that in C57BL/6J mice. The expression of p53 protein in adult naked mole rat was also significantly higher than that of the young. And high expression of p53 protein was detected in the naked mole rat fibroblast cells under hypoxia. Conclusion The expression level of tumor suppressor gene p53 protein in tissues of naked mole rats was significantly lower than that of C57BL/6J mice, and significant differences of p53 expression in different tissues of naked mole rats in different tissues, which may provide autoregulation mechanism ensuring an equilibrated expression of p53 gene.

关 键 词：裸鼹鼠 组织 P53基因 低氧 

分 类 号：R730[医药卫生—肿瘤] R-332[医药卫生—临床医学]