不同光质对小球藻光自养培养积累油脂的影响  

Effect of light wavelengths on lipid accumulation of Chlorella vulgaris in photoautotrophic culture

作　　者：尹继龙[1] 唐小红[1] 郑洪立[1] 尹丰伟[1] 高振[1] 黄和[1] 

机构地区：[1]南京工业大学生物与制药工程学院,南京211800

出　　处：《生物加工过程》2014年第5期62-68,共7页Chinese Journal of Bioprocess Engineering

基　　金：国家重点基础研究发展计划(973计划)(2011CB200904);国家自然科学基金(20936002)

摘　　要：研究了5种光质对小球藻(Chlorella vulgaris)M209256生长和产油的影响。结果表明:蓝光为小球藻的最适生长和产油光质;与其他光质相比,蓝光培养的小球藻生物量和油脂含量均较高,为2.40×107个/mL和28%;红光培养的小球藻生长最慢且油脂含量最低,为1.32×107个/mL和15.13%,表现出明显的"红降"现象。在GCMS分析的基础上,对油脂甲酯化后的十六烷值进行评估,结果发现:蓝光的十六烷值最高;5种光质培养的小球藻所产油脂,甲酯十六烷值均在47以上。因此,小球藻油脂所制备的生物柴油具有较好的燃烧性能。The effect of five kinds of light wavelengths on the growth and lipid production of Chlorella vulgaris M209256 was studied. The results showed that the optimal wavelength for growth and lipid production of Chlorella vulgaris was blue light. Compared with other light wavelengths,the higher biomass and lipid content of Chlorella vulgaris,2. 40 × 10^7mL^-1 and 28%,respectively,were obtained under blue light. Both the biomass and lipid content,1. 32 × 10^7mL^-1 and 15. 13%,respectively,were the lowest when Chlorella vulgaris was exposed to red light. It was a phenomenon of ＂ red drop＂. Cetane number（CN） was estimated in this work based on GC-MS analysis. It was found that Chlorella vulgaris under blue light had the highest CN and all CNs of fatty acid methyl esters（ FAMEs） from Chlorella vulgaris under the tested light wavelengths were above 47.

关 键 词：光质 小球藻 油脂含量 十六烷值 

分 类 号：Q819[生物学—生物工程]