沙利度胺对溃疡性结肠炎大鼠结肠组织MMP-9、COX-2、IL-8水平的影响  

Effect of thalidomide on the expression of MMP-9,COX-2 and IL-8 in rat model of ulcerative colitis

作　　者：段春兰[1] 宁宝森[1] 唐莉[1] 

机构地区：[1]内蒙古包头医学院第一附属医院消化科,内蒙古包头014010

出　　处：《中国中西医结合消化杂志》2014年第10期577-581,共5页Chinese Journal of Integrated Traditional and Western Medicine on Digestion

基　　金：内蒙古包头市卫生基金项目(No:Wsjj2013081)

摘　　要：[目的]探讨沙利度胺治疗溃疡性结肠炎大鼠的疗效及对结肠组织中基质金属蛋白酶-9(MMP-9)、环氧合酶-2(COX-2)、白介素-8(IL-8)表达水平的影响。[方法]将40只Wistar大鼠随机分为空白对照组、模型组、柳氮磺胺吡啶(SASP)组、沙利度胺组,每组10只。应用2,4,6三硝基苯磺酸与乙醇的混合物复制溃疡性结肠炎大鼠模型,除空白对照组和模型组正常饲养不进行药物治疗外,柳氮磺胺吡啶组给予SASP混悬液100mg/(kg·d)灌胃,沙利度胺组给予沙利度胺片混悬液200mg/(kg·d)灌胃。14d后处死全部大鼠,观察大鼠结肠组织大体形态、结肠炎症活动指数(DAI)评分和病理学的改变,并采用免疫组织化学法检测结肠组织中MMP-9、COX-2、IL-8的表达水平。[结果]沙利度胺能显著降低溃疡性结肠炎大鼠DAI评分和结肠组织中MMP-9、COX-2、IL-8的表达水平,并对损伤黏膜有较好的修复作用。[结论]沙利度胺对溃疡性结肠炎大鼠有较好的疗效,主要表现在促进黏膜愈合、降低组织中MMP-9、COX-2、IL-8的表达水平等方面。[Objective]To explore the eificacy of thalidomide in rat model of ulcerative colitis and the ex- pression of MMP-9, COX-2 and IL-8 in colon mucosa. [Methods]Forty Wistar rats were randomly divided into control group, model group, sulfaaalazine group and thalidomide group, every group containing 10 rats. TNBS and alcohol mixture were used to build the model o{ ulcerative colitis. Rats of sulfasalazine group were given orally 100 mg/（kg· d）. Rats of thalidomide group were given orally 200 mg/（kg·d）. The rats of control group and model group were fed with normal feeding without any drug. All rats were killed after 14 days. The general morphology of colon tissue,DAI score and pathology changes were ob- served. The expression of MMP-9, COX-2 and IL-8 in colon raucosa was detected by immunohistochemis- try. [Results]Thalidomide significantly reduced the DAI score and the expression of MMP-9, COX-2 and IL-8 in colon mucosa. Simultaneously,it played a role in repairing mucosal injury. [Conclusion]Thalidomide has a good effect on rat model of ulcerative colitis, mainly in promoting mucosal healing and reducing the expression of MMP-9, COX-2 and IL-8 in colon mucosa.

关 键 词：沙利度胺 溃疡性结肠炎 基质金属蛋白酶-9 环氧合酶-2 白介素-8 

分 类 号：R574.62[医药卫生—消化系统]