建立科研型肝癌肝移植标本库质量控制体系的初步探讨  

To establish quality control system of research-based clinical transplantation specimens bank for hepatocellular carcinoma

作　　者：王乐天[1] 张庆[1] 郝昱文[2] 陈虹[1] 陈新国[1] 沈中阳[1] 

机构地区：[1]武警总医院肝移植研究所,北京100039 [2]武警总医院计算机管理中心,北京100039

出　　处：《武警医学》2014年第10期987-990,共4页Medical Journal of the Chinese People's Armed Police Force

基　　金：国家自然科学基金项目(81372595);首都临床特色应用研究项目(Z121107001012168);武警总医院院内资助项目二类(WZ2010027)

摘　　要：目的探讨建立科研型肝癌肝移植临床标本库质量控制体系,制定规范化的肝癌肝移植标本采集、处理、保存操作流程及标本信息化管理系统。方法借鉴国内外建立标本库的标准化程序,制定肝癌肝移植标本库操作流程及质量控制体系,规范化收集、处理及保存肝癌肝移植受者的肿瘤组织及血液标本,同时收集良性肝病肝移植受者以及健康供者的肝组织及血液标本作为研究对照。对所收集的标本及相关的临床资料信息进行系统化管理。结果自2009-08肝癌肝移植标本库现收集有完整临床资料的501例肝移植供、受者的组织及术前/术后血液标本。其中,肝癌标本包括肿瘤组织、癌旁组织及远端非癌组织标本共计1773份。随机抽取45例标本进行质量监测,显示标本取材的准确率达90%以上,标本质量较好。开发了规范化的标本信息资料计算机管理系统。结论初步建立了具有一定规模的科研型肝癌肝移植标本库质量控制体系,有利于提高标本的质量,为肝移植研究提供了规范化的临床样本资源平台。Objective To study the quality control system for establishment of liver transplantation（ LT） biological specimen （ bio-specimens） bank for hepatocellular carcinoma（ HCC） , development of a standardized HCC for LT bio-specimens collection, pro-cessing and preservation operation process.To establish at sound and comprehensive information management system to conduct a sys-tematic management of clinical information of collected specimens.Methods Referring to standardized procedures to establish bio-specimens banks, the operational processes system in HCC for LT sample collection and quality control system were developed and standardized.Liver tissue and blood samples of HCC patients for LT were collected, While liver tissue and blood samples of benign liv-er disease in liver transplant recipients and of healthy donor were collected as a research control.A systematic management of clinical information of specimens collected was conducted.Results From the development of standardized sample collection procedures, in August 2009, and from 501 recipients and donors for LT, specimens of tissues and blood samples were collected.The total number of HCC specimens including tumor tissues, adjacent tissues and remote non-cancerous tissue specimens was 1773.And 45 were randomly selected for DNA and RNA testing;the results proved that the accuracy rate of pathological sampling was above than 90% and these collected specimens had a high quality.HCC specimen information data computer management was established and improved.Conclu-sions The initial establishment of standardized research-based clinical transplantation specimens bank for HCC is helpful to improving the efficiency of sample collection and quality.It provides a standardized samples resources platform for scientific research in the field.

关 键 词：肝细胞肝癌 肝移植 生物样本库 规范化收集 信息化管理 

分 类 号：R735.3[医药卫生—肿瘤]